These studies were designed to determine the effect of methylene blue on glutamate release and cGMP elevation. Methylene blue (10-1,000 μM) elevates the extracellular glutamate accumulation in a dose-dependent manner in cultured cerebellar neurons. Super oxide dismutase (SOD, 50 U mL-1 does not prevent extracellular glutamate accumulation induced by methylene blue or a reducing agent, dithiothreitol (DTT) while SOD suppresses oxygen radical generator (pyrogallol)-induced glutamate accumulation. Cyclic GMP level was highly elevated by sodium nitroprusside (SNP) but not changed by either methylene blue or DTT while the SNP-induced cGMP elevation was strongly prevented by both methylene blue and DTT. Collectively, these results indicate that methylene blue, independently of cGMP elevation, affects glutamate release as a reducing agent.
|Number of pages||3|
|Journal||Medical Science Research|
|State||Published - 1996|
- Cerebellar granule neuron
- Methylene blue