Metabolite profiling of red and white pitayas (Hylocereus polyrhizus and Hylocereus undatus) for comparing betalain biosynthesis and antioxidant activity

Sarah Lee, Dong Ho Suh, Sunmin Lee, Do Yeon Heo, Young Suk Kim, Somi Kim Cho, Choong Hwan Lee

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Metabolite profiling of red and white pitayas (Hylocereus polyrhizus and Hylocereus undatus) was performed using gas chromatography-time-of-flight-mass spectrometry and ultraperformance liquid chromatography-quadrupole-time-of- flight-mass spectrometry with multivariate analysis. Different species and parts of pitayas (red peel, RP; white peel, WP; red flesh, RF; and white flesh, WF) were clearly separated by partial least-squares discriminate analysis. Furthermore, betalain-related metabolites, such as betacyanins and betaxanthins, or their precursors were described on the basis of their metabolites. The results of antioxidant activity tests [1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and ferric reducing ability of plasma (FRAP)], total phenolic contents (TPC), total flavonoid contents (TFC), and total betacyanin contents (TBC) showed the following: RP ≥ WP > RF > WF. TPC, TFC, TBC, and betalain-related metabolites were higher in the peel than in the flesh and suggested to be the main contributors to antioxidant activity in pitayas. Therefore, peels as well as pulp of pitaya could beneficially help in the food industry.

Original languageEnglish
Pages (from-to)8764-8771
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number34
DOIs
StatePublished - 27 Aug 2014

Keywords

  • antioxidant activity
  • betalain biosynthesis pathway
  • GC-TOF-MS
  • Hylocereus polyrhizus
  • Hylocereus undatus
  • metabolite profiling
  • UPLC-Q-TOF-MS

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