Metabolite profiling of doenjang, fermented soybean paste, during fermentation

Hye Jung Namgung, Hye Jung Park, In Hee Cho, Hyung Kyoon Choi, Dae Young Kwon, Soon Mi Shim, Young Suk Kim

Research output: Contribution to journalArticlepeer-review

102 Scopus citations


BACKGROUND: A fermented soybean paste known as doenjang is a traditional fermented food that is widely consumed in Korea. The quality of doenjang varies considerably by its basic ingredients, species of microflora, and fermentation process. The classification of predefined metabolites (e.g. amino acids, organic acids, sugars and sugar derivatives, and fatty acids) in doenjang samples according to fermentation was performed by using GC-FID and GC-MS data sets with the application of a multivariate statisticalmethod. RESULTS: The predominantly produced amino acids included alanine, valine, leucine, isoleucine, proline, glutamine, phenylalanine and lysine, showing remarkable increases in amounts during the later stages of fermentation. Carbonic acid, citric acid, lactic acid and pyrogultamic acid were identified as the major organic acids. Significant amounts of erythrose, xylitol, inositol and mannitol were detected during fermentation. Regarding fatty acids, relatively higher amounts of palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acidwere found in the doenjang at each fermentation time point. Principal component analysis (PCA) successfully demonstrated changes in composition patterns as well as differences in non-volatile metabolites according to fermentation period. CONCLUSION: A set ofmetabolites could be determined representing the quality of doenjang during fermentation, and which might also be correlated with taste ingredients, flavour, nutrition, and physiology activities that are claimed to be dependent on the quality control of commercial doenjang.

Original languageEnglish
Pages (from-to)1926-1935
Number of pages10
JournalJournal of the Science of Food and Agriculture
Issue number11
StatePublished - 30 Aug 2010


  • Amino acids
  • Fatty acids
  • Fermented soybean paste
  • GC-MS
  • Metabolites
  • Organic acids
  • PCA
  • Sugars and sugar derivatives


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