Metabolic engineering of Escherichia coli for the production of medium-chain-length polyhydroxyalkanoates rich in specific monomers

Si Jae Park; Jong Pil Park; Sang Yup Lee

doi:10.1016/S0378-1097(02)00888-1

Metabolic engineering of Escherichia coli for the production of medium-chain-length polyhydroxyalkanoates rich in specific monomers

Si Jae Park, Jong Pil Park, Sang Yup Lee

Department of Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

The Escherichia coli fabG_Ec gene and the Pseudomonas aeruginosa rhlG_Pa gene, which encode 3-ketoacyl-acyl carrier protein reductase, were expressed in E. coli W3110 and its fadA mutant strain WA101 to examine their roles in medium-chain-length (MCL) polyhydroxyalkanoate (PHA) biosynthesis from fatty acids. When one of these 3-ketoacyl-acyl carrier protein reductase genes was co-expressed with the Pseudomonas sp. 61-3 PHA synthase gene (phaC2_Ps) in E. coli W3110, MCL-PHA composed mainly of 3-hydroxyoctanoate and 3-hydroxydecanoate was synthesized from sodium decanoate. When the fabG_Ec gene and the phaC2_Ps gene were co-expressed in the fadA mutant E. coli strain WA101, MCL-PHA rich in 3-hydroxydecanoate monomer up to 93 mol% was accumulated from sodium decanoate. This was possible by efficiently redirecting 3-ketoacyl-coenzymes A from the β-oxidation pathway to the PHA biosynthesis pathway without losing two carbon units, the strategy of which can be extended for the production of MCL-PHAs rich in other specific monomers.

Original language	English
Pages (from-to)	217-222
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	214
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(02)00888-1
State	Published - 10 Sep 2002

Bibliographical note

Funding Information:
This work was supported by the National Research Laboratory Program (2000-N-NL-01-C-237) of the Korean Ministry of Science and Technology (MOST). We thank Dr. Y. Doi and Dr. Isabelle-S. Hinner for kindly providing us with plasmid pBSEB50 and pBBR1MCS, respectively. We also thank Dr. G.M. Church for the kind gift of plasmid pKO3.

Keywords

β-Oxidation pathway
3-Ketoacyl-acyl carrier protein reductase
Escherichia coli
Escherichia coli FabG
Polyhydroxyalkanoate
Pseudomonas aeruginosa RhlG

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10.1016/S0378-1097(02)00888-1

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title = "Metabolic engineering of Escherichia coli for the production of medium-chain-length polyhydroxyalkanoates rich in specific monomers",

abstract = "The Escherichia coli fabGEc gene and the Pseudomonas aeruginosa rhlGPa gene, which encode 3-ketoacyl-acyl carrier protein reductase, were expressed in E. coli W3110 and its fadA mutant strain WA101 to examine their roles in medium-chain-length (MCL) polyhydroxyalkanoate (PHA) biosynthesis from fatty acids. When one of these 3-ketoacyl-acyl carrier protein reductase genes was co-expressed with the Pseudomonas sp. 61-3 PHA synthase gene (phaC2Ps) in E. coli W3110, MCL-PHA composed mainly of 3-hydroxyoctanoate and 3-hydroxydecanoate was synthesized from sodium decanoate. When the fabGEc gene and the phaC2Ps gene were co-expressed in the fadA mutant E. coli strain WA101, MCL-PHA rich in 3-hydroxydecanoate monomer up to 93 mol% was accumulated from sodium decanoate. This was possible by efficiently redirecting 3-ketoacyl-coenzymes A from the β-oxidation pathway to the PHA biosynthesis pathway without losing two carbon units, the strategy of which can be extended for the production of MCL-PHAs rich in other specific monomers.",

keywords = "β-Oxidation pathway, 3-Ketoacyl-acyl carrier protein reductase, Escherichia coli, Escherichia coli FabG, Polyhydroxyalkanoate, Pseudomonas aeruginosa RhlG",

author = "Park, {Si Jae} and Park, {Jong Pil} and Lee, {Sang Yup}",

note = "Funding Information: This work was supported by the National Research Laboratory Program (2000-N-NL-01-C-237) of the Korean Ministry of Science and Technology (MOST). We thank Dr. Y. Doi and Dr. Isabelle-S. Hinner for kindly providing us with plasmid pBSEB50 and pBBR1MCS, respectively. We also thank Dr. G.M. Church for the kind gift of plasmid pKO3.",

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AU - Park, Si Jae

AU - Park, Jong Pil

AU - Lee, Sang Yup

N1 - Funding Information: This work was supported by the National Research Laboratory Program (2000-N-NL-01-C-237) of the Korean Ministry of Science and Technology (MOST). We thank Dr. Y. Doi and Dr. Isabelle-S. Hinner for kindly providing us with plasmid pBSEB50 and pBBR1MCS, respectively. We also thank Dr. G.M. Church for the kind gift of plasmid pKO3.

PY - 2002/9/10

Y1 - 2002/9/10

N2 - The Escherichia coli fabGEc gene and the Pseudomonas aeruginosa rhlGPa gene, which encode 3-ketoacyl-acyl carrier protein reductase, were expressed in E. coli W3110 and its fadA mutant strain WA101 to examine their roles in medium-chain-length (MCL) polyhydroxyalkanoate (PHA) biosynthesis from fatty acids. When one of these 3-ketoacyl-acyl carrier protein reductase genes was co-expressed with the Pseudomonas sp. 61-3 PHA synthase gene (phaC2Ps) in E. coli W3110, MCL-PHA composed mainly of 3-hydroxyoctanoate and 3-hydroxydecanoate was synthesized from sodium decanoate. When the fabGEc gene and the phaC2Ps gene were co-expressed in the fadA mutant E. coli strain WA101, MCL-PHA rich in 3-hydroxydecanoate monomer up to 93 mol% was accumulated from sodium decanoate. This was possible by efficiently redirecting 3-ketoacyl-coenzymes A from the β-oxidation pathway to the PHA biosynthesis pathway without losing two carbon units, the strategy of which can be extended for the production of MCL-PHAs rich in other specific monomers.

AB - The Escherichia coli fabGEc gene and the Pseudomonas aeruginosa rhlGPa gene, which encode 3-ketoacyl-acyl carrier protein reductase, were expressed in E. coli W3110 and its fadA mutant strain WA101 to examine their roles in medium-chain-length (MCL) polyhydroxyalkanoate (PHA) biosynthesis from fatty acids. When one of these 3-ketoacyl-acyl carrier protein reductase genes was co-expressed with the Pseudomonas sp. 61-3 PHA synthase gene (phaC2Ps) in E. coli W3110, MCL-PHA composed mainly of 3-hydroxyoctanoate and 3-hydroxydecanoate was synthesized from sodium decanoate. When the fabGEc gene and the phaC2Ps gene were co-expressed in the fadA mutant E. coli strain WA101, MCL-PHA rich in 3-hydroxydecanoate monomer up to 93 mol% was accumulated from sodium decanoate. This was possible by efficiently redirecting 3-ketoacyl-coenzymes A from the β-oxidation pathway to the PHA biosynthesis pathway without losing two carbon units, the strategy of which can be extended for the production of MCL-PHAs rich in other specific monomers.

KW - β-Oxidation pathway

KW - 3-Ketoacyl-acyl carrier protein reductase

KW - Escherichia coli

KW - Escherichia coli FabG

KW - Polyhydroxyalkanoate

KW - Pseudomonas aeruginosa RhlG

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SN - 0378-1097

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EP - 222

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

Metabolic engineering of Escherichia coli for the production of medium-chain-length polyhydroxyalkanoates rich in specific monomers

Abstract

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Keywords

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