Metabolic engineering of Escherichia coli for biosynthesis of D-galactonate

Huaiwei Liu, Kristine Rose M. Ramos, Kris Niño G. Valdehuesa, Grace M. Nisola, Lenny B. Malihan, Won Keun Lee, Si Jae Park, Wook Jin Chung

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

D-galactose is an attractive substrate for bioconversion. Herein, Escherichia coli was metabolically engineered to convert D-galactose into D-galactonate, a valuable compound in the polymer and cosmetic industries. D-galactonate productions by engineered E. coli strains were observed in shake flask cultivations containing 2 g L-1 D-galactose. Engineered E. coli expressing gld coding for galactose dehydrogenase from Pseudomonas syringae was able to produce 0.17 g L-1 D-galactonate. Inherent metabolic pathways for assimilating both D-galactose and D-galactonate were blocked to enhance the production of D-galactonate. This approach finally led to a 7.3-fold increase with D-galactonate concentration of 1.24 g L-1 and yield of 62.0 %. Batch fermentation in 20 g L-1 D-galactose of E. coli ΔgalKΔdgoK mutant expressing the gld resulted in 17.6 g L -1 of D-galactonate accumulation and highest yield of 88.1 %. Metabolic engineering strategy developed in this study could be useful for industrial production of D-galactonate.

Original languageEnglish
Pages (from-to)383-391
Number of pages9
JournalBioprocess and Biosystems Engineering
Volume37
Issue number3
DOIs
StatePublished - Mar 2014

Keywords

  • Bioconversion
  • D-galactonate
  • D-galactose
  • Galactose dehydrogenase
  • Metabolic engineering

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