Metabolic engineering of Escherichia coli for biosynthesis of D-galactonate

Huaiwei Liu; Kristine Rose M. Ramos; Kris Niño G. Valdehuesa; Grace M. Nisola; Lenny B. Malihan; Won Keun Lee; Si Jae Park; Wook Jin Chung

doi:10.1007/s00449-013-1003-6

Metabolic engineering of Escherichia coli for biosynthesis of D-galactonate

Huaiwei Liu, Kristine Rose M. Ramos, Kris Niño G. Valdehuesa, Grace M. Nisola, Lenny B. Malihan, Won Keun Lee, Si Jae Park, Wook Jin Chung

Department of Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

D-galactose is an attractive substrate for bioconversion. Herein, Escherichia coli was metabolically engineered to convert D-galactose into D-galactonate, a valuable compound in the polymer and cosmetic industries. D-galactonate productions by engineered E. coli strains were observed in shake flask cultivations containing 2 g L^-1 D-galactose. Engineered E. coli expressing gld coding for galactose dehydrogenase from Pseudomonas syringae was able to produce 0.17 g L^-1 D-galactonate. Inherent metabolic pathways for assimilating both D-galactose and D-galactonate were blocked to enhance the production of D-galactonate. This approach finally led to a 7.3-fold increase with D-galactonate concentration of 1.24 g L^-1 and yield of 62.0 %. Batch fermentation in 20 g L^-1 D-galactose of E. coli ΔgalKΔdgoK mutant expressing the gld resulted in 17.6 g L ^-1 of D-galactonate accumulation and highest yield of 88.1 %. Metabolic engineering strategy developed in this study could be useful for industrial production of D-galactonate.

Original language	English
Pages (from-to)	383-391
Number of pages	9
Journal	Bioprocess and Biosystems Engineering
Volume	37
Issue number	3
DOIs	https://doi.org/10.1007/s00449-013-1003-6
State	Published - Mar 2014

Bibliographical note

Funding Information:
Acknowledgments This work was supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012-0006693).

Keywords

Bioconversion
D-galactonate
D-galactose
Galactose dehydrogenase
Metabolic engineering

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10.1007/s00449-013-1003-6

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title = "Metabolic engineering of Escherichia coli for biosynthesis of D-galactonate",

abstract = "D-galactose is an attractive substrate for bioconversion. Herein, Escherichia coli was metabolically engineered to convert D-galactose into D-galactonate, a valuable compound in the polymer and cosmetic industries. D-galactonate productions by engineered E. coli strains were observed in shake flask cultivations containing 2 g L-1 D-galactose. Engineered E. coli expressing gld coding for galactose dehydrogenase from Pseudomonas syringae was able to produce 0.17 g L-1 D-galactonate. Inherent metabolic pathways for assimilating both D-galactose and D-galactonate were blocked to enhance the production of D-galactonate. This approach finally led to a 7.3-fold increase with D-galactonate concentration of 1.24 g L-1 and yield of 62.0 %. Batch fermentation in 20 g L-1 D-galactose of E. coli ΔgalKΔdgoK mutant expressing the gld resulted in 17.6 g L -1 of D-galactonate accumulation and highest yield of 88.1 %. Metabolic engineering strategy developed in this study could be useful for industrial production of D-galactonate.",

keywords = "Bioconversion, D-galactonate, D-galactose, Galactose dehydrogenase, Metabolic engineering",

author = "Huaiwei Liu and Ramos, {Kristine Rose M.} and Valdehuesa, {Kris Ni{\~n}o G.} and Nisola, {Grace M.} and Malihan, {Lenny B.} and Lee, {Won Keun} and Park, {Si Jae} and Chung, {Wook Jin}",

note = "Funding Information: Acknowledgments This work was supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012-0006693).",

year = "2014",

month = mar,

doi = "10.1007/s00449-013-1003-6",

language = "English",

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pages = "383--391",

journal = "Bioprocess and Biosystems Engineering",

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AU - Liu, Huaiwei

AU - Ramos, Kristine Rose M.

AU - Valdehuesa, Kris Niño G.

AU - Nisola, Grace M.

AU - Malihan, Lenny B.

AU - Lee, Won Keun

AU - Park, Si Jae

AU - Chung, Wook Jin

N1 - Funding Information: Acknowledgments This work was supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012-0006693).

PY - 2014/3

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N2 - D-galactose is an attractive substrate for bioconversion. Herein, Escherichia coli was metabolically engineered to convert D-galactose into D-galactonate, a valuable compound in the polymer and cosmetic industries. D-galactonate productions by engineered E. coli strains were observed in shake flask cultivations containing 2 g L-1 D-galactose. Engineered E. coli expressing gld coding for galactose dehydrogenase from Pseudomonas syringae was able to produce 0.17 g L-1 D-galactonate. Inherent metabolic pathways for assimilating both D-galactose and D-galactonate were blocked to enhance the production of D-galactonate. This approach finally led to a 7.3-fold increase with D-galactonate concentration of 1.24 g L-1 and yield of 62.0 %. Batch fermentation in 20 g L-1 D-galactose of E. coli ΔgalKΔdgoK mutant expressing the gld resulted in 17.6 g L -1 of D-galactonate accumulation and highest yield of 88.1 %. Metabolic engineering strategy developed in this study could be useful for industrial production of D-galactonate.

AB - D-galactose is an attractive substrate for bioconversion. Herein, Escherichia coli was metabolically engineered to convert D-galactose into D-galactonate, a valuable compound in the polymer and cosmetic industries. D-galactonate productions by engineered E. coli strains were observed in shake flask cultivations containing 2 g L-1 D-galactose. Engineered E. coli expressing gld coding for galactose dehydrogenase from Pseudomonas syringae was able to produce 0.17 g L-1 D-galactonate. Inherent metabolic pathways for assimilating both D-galactose and D-galactonate were blocked to enhance the production of D-galactonate. This approach finally led to a 7.3-fold increase with D-galactonate concentration of 1.24 g L-1 and yield of 62.0 %. Batch fermentation in 20 g L-1 D-galactose of E. coli ΔgalKΔdgoK mutant expressing the gld resulted in 17.6 g L -1 of D-galactonate accumulation and highest yield of 88.1 %. Metabolic engineering strategy developed in this study could be useful for industrial production of D-galactonate.

KW - Bioconversion

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KW - D-galactose

KW - Galactose dehydrogenase

KW - Metabolic engineering

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Metabolic engineering of Escherichia coli for biosynthesis of D-galactonate

Abstract

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Keywords

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