Metabolic Engineering of Corynebacterium glutamicum for the High-Level Production of Cadaverine That Can Be Used for the Synthesis of Biopolyamide 510

Hee Taek Kim, Kei Anne Baritugo, Young Hoon Oh, Sung Min Hyun, Tae Uk Khang, Kyoung Hee Kang, Sol Hee Jung, Bong Keun Song, Kyungmoon Park, Il Kwon Kim, Myung Ock Lee, Yeji Kam, Yong Taek Hwang, Si Jae Park, Jeong Chan Joo

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63 Scopus citations

Abstract

Fermentative production of cadaverine from renewable resources may support a sustainable biorefinery process to produce carbon-neutral nylons such as biopolyamide 510 (PA510). Cost-competitive production of cadaverine is a key factor in the successful commercialization of PA510. In this study, an integrated biological and chemical process involving cadaverine biosynthesis, purification, and its polymerization with sebacic acid was developed to produce bio-PA510. To stably express ldcC from Escherichia coli in an engineered Corynebacterium glutamicum PKC strain, an expired industrial l-lysine-producing strain, ldcC, was integrated into the chromosome of the C. glutamicum PKC strain by disrupting lysE and controlling its expression via a strong synthetic H30 promoter. Cadaverine was produced at a concentration of 103.78 g/L, the highest titer to date, from glucose by fed-batch culture of this engineered C. glutamgicum PKC strain. Fermentation-derived cadaverine was purified to polymer-grade biocadaverine with high purity (99%) by solvent extraction with chloroform and two-step distillation. Finally, biobased PA510 with good thermal properties (Tm 215 °C and Tc 158 °C) was produced by polymerization of purified cadaverine with sebacic acid. The hybrid biorefinery process combining biological and chemical processes demonstrated in this study is a useful platform for producing biobased chemicals and polymers.

Original languageEnglish
Pages (from-to)5296-5305
Number of pages10
JournalACS Sustainable Chemistry and Engineering
Volume6
Issue number4
DOIs
StatePublished - 2 Apr 2018

Keywords

  • Biopolyamide
  • Cadaverine
  • L-Lysine
  • Lysine decarboxylase
  • PA510
  • Recombinant Corynebacterium glutamicum

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