Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow

Kyung Ah Cho, Sun Young Ju, Su Jin Cho, Yun Jae Jung, So Youn Woo, Ju Young Seoh, Ho Seong Han, Kyung Ha Ryu

Research output: Contribution to journalArticlepeer-review

85 Scopus citations

Abstract

We have previously reported that bone marrow cells (BMCs) participate in the regeneration after liver injury. However, it is not established that this is the result of differentiation of hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) or the combination of both. We investigated the contribution of each cell fraction to the regenerative process. First, we confirmed that transplanted stem cells migrate directly to injured liver tissue without dispersing to other organs. Next, we divided green fluorescent protein (GFP)-expressing BMCs into three populations as mononuclear cells, MSCs and HSCs. We then compared the engraftment capacity after transplantation of each fraction of cells into liver-injured mice. Of these, the MSCs transplanted group showed the highest GFP fluorescence intensities in liver tissue by flow cytometry analysis and confocal microscopic observation. Furthermore, MSCs showed differentiation potential into hepatocytes when co-cultured with injured liver cells, which suggests that MSCs showed highest potential for the regeneration of injured liver tissue compared with those of the other two cell refractions.

Original languageEnglish
Pages (from-to)772-777
Number of pages6
JournalCell Biology International
Volume33
Issue number7
DOIs
StatePublished - Jul 2009

Keywords

  • Liver injury
  • Mesenchymal stem cells (MSCs)
  • Transplantation

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