Melatonin and taurine reduce early glomerulopathy in diabetic rats

Hunjoo Ha, Mi Ra Yu, Kyung Hwan Kim

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139 Scopus citations

Abstract

Oxidative stress occurs in diabetic patients and experimental models of diabetes. We examined whether two antioxidants, melatonin and taurine, can ameliorate diabetic nephropathy. Enhanced expression of glomerular TGF-β1 and fibronectin mRNAs and proteinuria were employed as indices of diabetic nephropathy. Experimental diabetes was induced by intravenous injection of streptozotocin 50 mg/kg. Two days after streptozotocin, diabetic rats were assigned to one of the following groups: i) untreated; ii) melatonin supplement by 0.02% in drinking water; or iii) taurine supplement by 1% in drinking water. Four weeks after streptozotocin, diabetic rats (n = 6: plasma glucose 516 ± 12 mg/dl) exhibited 6.1 fold increase in urinary protein excretion, 1.4 fold increase in glomerular TGF-β1 mRNA, 1.7 fold increase in glomerular fibronectin mRNA, 2.2 fold increase in plasma lipid peroxides (LPO), and 44 fold increase in urinary LPO excretion above the values in control rats (n = 6: plasma glucose 188 ± 14 mg/dl). Chronic administration of melatonin (n = 6) and taurine (n = 6) prevented increases in glomerular TGF-β1 and fibronectin mRNAs and proteinuria without having effect on blood glucose. Both treatments reduced lipid peroxidation by nearly 50%. The present data demonstrate beneficial effects of melatonin and taurine on early changes in diabetic kidney and suggest that diabetic nephropathy associated with hyperglycemia is largely mediated by oxidative stress.

Original languageEnglish
Pages (from-to)944-950
Number of pages7
JournalFree Radical Biology and Medicine
Volume26
Issue number7-8
DOIs
StatePublished - Apr 1999

Bibliographical note

Funding Information:
We thank Professor Hi Bahl Lee at Hyonam Kidney Laboratory, Soon Chun Hyang University, for his continued interest in our work and constructive criticism and Dr. Sung Il Kim for providing cDNA probes for TGF-β1 and GAPDH. This work was supported in part by a grant from Korea Science and Engineering foundation (KOSEF 94-0403-07-01-3). Portions of this work were presented at the 30th Annual Meeting of American Society of Nephrology (San Antonio, TX, USA, 1997) and appeared in abstract form.

Keywords

  • Diabetic glomerulopathy
  • Fibronectin
  • Free radical
  • Melatonin
  • Oxidative stress
  • Streptozotocin
  • TGF- β1
  • Taurine

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