Mechanism by which hydrogen peroxide enhances intracellular calcium in A431 cells

Hae Won Won Yoon; Yun Soo Bae; Sue Goo Rhee

Mechanism by which hydrogen peroxide enhances intracellular calcium in A431 cells

Hae Won Won Yoon, Yun Soo Bae, Sue Goo Rhee

Department of Life Science

Research output: Contribution to journal › Article › peer-review

Abstract

It has been shown tht oxogenousty added H₂O₂ elicits tyrosine phosphorylation of a variety of protine including PLC as well as Ca²⁺ mobiliza tion in several cell types. We investigated the likage between PLC phosphorylation and CA²⁺ mobilization in A431 cells. Immunoblot analysis with anti-phospotyrosine antibilization incirated that the tyrosine phosphorylation of PLC peaked within 5 min after H₂O ₂ treatment and returned to basal level within 30 min. The PLC phosphorylation coincided with the onset of H₂O₂ treatment and returned to basal level with induced inositol phosphates production Prior incubation of A431 cells with thapsigargin caused a nearly complete inhibition of H₂O₂-dependent Ca²⁺ mobilization in the absence of extracellular CA²⁺. These results suggest that the phospherylation of PLC induced by H₂O₂ and subsequent product on of In-(1.4.5)P₃ are mainly responsible for the CA ²⁺ mobilization observed in A431 cels stimulated with H ₂O₂.

Original language	English
Pages (from-to)	A1356
Journal	FASEB Journal
Volume	11
Issue number	9
State	Published - 1997

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title = "Mechanism by which hydrogen peroxide enhances intracellular calcium in A431 cells",

abstract = "It has been shown tht oxogenousty added H2O2 elicits tyrosine phosphorylation of a variety of protine including PLC as well as Ca2+ mobiliza tion in several cell types. We investigated the likage between PLC phosphorylation and CA2+ mobilization in A431 cells. Immunoblot analysis with anti-phospotyrosine antibilization incirated that the tyrosine phosphorylation of PLC peaked within 5 min after H2O 2 treatment and returned to basal level within 30 min. The PLC phosphorylation coincided with the onset of H2O2 treatment and returned to basal level with induced inositol phosphates production Prior incubation of A431 cells with thapsigargin caused a nearly complete inhibition of H2O2-dependent Ca2+ mobilization in the absence of extracellular CA2+. These results suggest that the phospherylation of PLC induced by H2O2 and subsequent product on of In-(1.4.5)P3 are mainly responsible for the CA 2+ mobilization observed in A431 cels stimulated with H 2O2.",

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year = "1997",

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AU - Yoon, Hae Won Won

AU - Bae, Yun Soo

AU - Rhee, Sue Goo

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N2 - It has been shown tht oxogenousty added H2O2 elicits tyrosine phosphorylation of a variety of protine including PLC as well as Ca2+ mobiliza tion in several cell types. We investigated the likage between PLC phosphorylation and CA2+ mobilization in A431 cells. Immunoblot analysis with anti-phospotyrosine antibilization incirated that the tyrosine phosphorylation of PLC peaked within 5 min after H2O 2 treatment and returned to basal level within 30 min. The PLC phosphorylation coincided with the onset of H2O2 treatment and returned to basal level with induced inositol phosphates production Prior incubation of A431 cells with thapsigargin caused a nearly complete inhibition of H2O2-dependent Ca2+ mobilization in the absence of extracellular CA2+. These results suggest that the phospherylation of PLC induced by H2O2 and subsequent product on of In-(1.4.5)P3 are mainly responsible for the CA 2+ mobilization observed in A431 cels stimulated with H 2O2.

AB - It has been shown tht oxogenousty added H2O2 elicits tyrosine phosphorylation of a variety of protine including PLC as well as Ca2+ mobiliza tion in several cell types. We investigated the likage between PLC phosphorylation and CA2+ mobilization in A431 cells. Immunoblot analysis with anti-phospotyrosine antibilization incirated that the tyrosine phosphorylation of PLC peaked within 5 min after H2O 2 treatment and returned to basal level within 30 min. The PLC phosphorylation coincided with the onset of H2O2 treatment and returned to basal level with induced inositol phosphates production Prior incubation of A431 cells with thapsigargin caused a nearly complete inhibition of H2O2-dependent Ca2+ mobilization in the absence of extracellular CA2+. These results suggest that the phospherylation of PLC induced by H2O2 and subsequent product on of In-(1.4.5)P3 are mainly responsible for the CA 2+ mobilization observed in A431 cels stimulated with H 2O2.

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Mechanism by which hydrogen peroxide enhances intracellular calcium in A431 cells

Abstract

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