Mechanical cell lysis results of a sample preparation module for functional genomics

D. Di Carlo; L. P. Lee

doi:10.1109/MMB.2002.1002399

Mechanical cell lysis results of a sample preparation module for functional genomics

D. Di Carlo, L. P. Lee

Department of Chemistry & Nanoscience

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

6 Scopus citations

Abstract

Mechanical cell lysis is demonstrated within a microfabricated sample preparation module that would assist functional genomics and point-of-care diagnostics. The sample preparation module is proposed as a component of a vertically integrated system with RT-PCR and capillary gel electrophoresis modules. Cell lysis is conducted within the module using a micro-barbed mechanical filter within microfluidic channels. Cell behavior within the lysis filter is qualitatively described. The filter is microfabricated using a modified DRIE process that enhances the scalloping effect to create sub-micrometer scale barbs. An integrated mRNA affinity chamber is also developed with ssDNA immobilized on a silicon surface using a self-assembled monolayer linker.

Original language	English
Title of host publication	2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
Editors	David Beebe, Andre Dittmar
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	527-530
Number of pages	4
ISBN (Electronic)	0780374800, 9780780374805
DOIs	https://doi.org/10.1109/MMB.2002.1002399
State	Published - 2002
Event	2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Madison, United States Duration: 2 May 2002 → 4 May 2002

Publication series

Name	2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings

Conference

Conference	2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology
Country/Territory	United States
City	Madison
Period	2/05/02 → 4/05/02

Bibliographical note

Publisher Copyright:
© 2002 IEEE.

Access to Document

10.1109/MMB.2002.1002399

Cite this

Di Carlo, D., & Lee, L. P. (2002). Mechanical cell lysis results of a sample preparation module for functional genomics. In D. Beebe, & A. Dittmar (Eds.), 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings (pp. 527-530). Article 1002399 (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MMB.2002.1002399

Di Carlo, D. ; Lee, L. P. / Mechanical cell lysis results of a sample preparation module for functional genomics. 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. editor / David Beebe ; Andre Dittmar. Institute of Electrical and Electronics Engineers Inc., 2002. pp. 527-530 (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings).

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abstract = "Mechanical cell lysis is demonstrated within a microfabricated sample preparation module that would assist functional genomics and point-of-care diagnostics. The sample preparation module is proposed as a component of a vertically integrated system with RT-PCR and capillary gel electrophoresis modules. Cell lysis is conducted within the module using a micro-barbed mechanical filter within microfluidic channels. Cell behavior within the lysis filter is qualitatively described. The filter is microfabricated using a modified DRIE process that enhances the scalloping effect to create sub-micrometer scale barbs. An integrated mRNA affinity chamber is also developed with ssDNA immobilized on a silicon surface using a self-assembled monolayer linker.",

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Di Carlo, D & Lee, LP 2002, Mechanical cell lysis results of a sample preparation module for functional genomics. in D Beebe & A Dittmar (eds), 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings., 1002399, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings, Institute of Electrical and Electronics Engineers Inc., pp. 527-530, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, Madison, United States, 2/05/02. https://doi.org/10.1109/MMB.2002.1002399

Mechanical cell lysis results of a sample preparation module for functional genomics. / Di Carlo, D.; Lee, L. P.
2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. ed. / David Beebe; Andre Dittmar. Institute of Electrical and Electronics Engineers Inc., 2002. p. 527-530 1002399 (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AB - Mechanical cell lysis is demonstrated within a microfabricated sample preparation module that would assist functional genomics and point-of-care diagnostics. The sample preparation module is proposed as a component of a vertically integrated system with RT-PCR and capillary gel electrophoresis modules. Cell lysis is conducted within the module using a micro-barbed mechanical filter within microfluidic channels. Cell behavior within the lysis filter is qualitatively described. The filter is microfabricated using a modified DRIE process that enhances the scalloping effect to create sub-micrometer scale barbs. An integrated mRNA affinity chamber is also developed with ssDNA immobilized on a silicon surface using a self-assembled monolayer linker.

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Di Carlo D, Lee LP. Mechanical cell lysis results of a sample preparation module for functional genomics. In Beebe D, Dittmar A, editors, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2002. p. 527-530. 1002399. (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings). doi: 10.1109/MMB.2002.1002399

Mechanical cell lysis results of a sample preparation module for functional genomics

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