Mechanical and mechanosensing properties of tumor affected bone cells were inhibited via PI3K/AKt pathway

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Background: Osteolytic metastasis is a common destructive form of metastasis, in which there is an increased bone resorption but impaired bone formation. It is hypothesized that the changed mechanical properties of tumor affected bone cells could inhibit its mechanosensing, thus contributing to differences in bone remodeling. Methods: Here, atomic force microscopy indentation on primary bone cells exposed to 50% conditioned medium from Walker 256 (W) carcinoma cell line or its adaptive tumor (T) cells was carried out. Nitric oxide levels of bone cells were monitored in response to low-magnitude, high- frequency (LMHF) vibrations. Results: A stronger sustained inhibitive effect on bone cell viability and differentiation by T cells as compared to that of its cell line was demonstrated. This could be attributed to the higher levels of transforming growth factor-β1 (TGF-β1) in the T-conditioned medium as compared to W-conditioned medium. Bone cell elastic moduli in W and T-groups were found to decrease significantly by 61.0% and 69.6%, respectively compared to control and corresponded to filamentous actin changes. Nitric oxide responses were significantly inhibited in T-conditioned group but not in W-conditioned group. Conclusions: It implied that a change in cell mechanical properties is not sufficient as an indicator of change in mechanosensing ability. Moreover, inhibition of phosphoinositide 3-kinase/Akt downstream signaling pathway of TGF-β1 alleviated the inhibition effects on mechanosensing in T-conditioned cells, further suggesting that growth factors such as TGF-β could be good therapeutic targets for osteoblast treatment.

Original languageEnglish
Pages (from-to)179-191
Number of pages13
JournalJournal of Bone Metabolism
Issue number3
StatePublished - 1 Aug 2019

Bibliographical note

Publisher Copyright:
Copyright © 2019 The Korean Society for Bone and Mineral Research.


  • Bone metastasis
  • Bone neoplasms
  • Elastic modulus
  • Mechanosensing
  • Nitric oxide


Dive into the research topics of 'Mechanical and mechanosensing properties of tumor affected bone cells were inhibited via PI3K/AKt pathway'. Together they form a unique fingerprint.

Cite this