Measurement of phosphorylated phospholamban levels in cardiomyocytes (HL-1) by immunoprecipitation.

C. V. Suresh Babu, Eun Joo Song, Sheikh Md Enayetul Babar, Young Sook Yoo

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Phospholamban (PLN) is a key regulatory protein involved in cardiac calcium signaling through the pumping of cytoplasmic Ca2+ into the sarcoplasmic reticulum (SR). Recent systems-level studies have focused on integrating quantitative data (e.g. protein expression levels) for a better understanding of cardiac systems biology. In this view, we developed a capillary electrophoresis (CE) based immunoprecipitation method for the measurement of phospho-PLN (ser 16) in cardiomyocytes (HL-1 cell line). Dose-dependent isoproterenol (Iso) treated cells were analyzed using CE, and the phospho-PLN levels were quantified using specific polyclonal antibodies. The CE method employed was accurate, quick and easier compare to other techniques and the results are useful for the subsequent computational systems biology research.

Original languageEnglish
Pages (from-to)838-844
Number of pages7
JournalJournal of Biochemical and Biophysical Methods
Issue number6
StatePublished - 24 Apr 2008

Bibliographical note

Funding Information:
The authors wish to thank Mrs Anne J. Charlton for English proof reading and helpful comments on the manuscript. This work was supported by the grants from the Korea Ministry of Science and Technology (Systems Biology Research Grant; 2N29550).


Dive into the research topics of 'Measurement of phosphorylated phospholamban levels in cardiomyocytes (HL-1) by immunoprecipitation.'. Together they form a unique fingerprint.

Cite this