Matrix metalloproteinase-2, membranous type 1 matrix metalloproteinase, and tissue inhibitor of metalloproteinase-2 expression in ectopic and eutopic endometrium

Hye Won Chung, Ji Young Lee, Hye Sung Moon, Sung Eun Hur, Mi Hye Park, Yan Wen, Mary Lake Polan

Research output: Contribution to journalArticlepeer-review

157 Scopus citations

Abstract

Objective: To investigate expression of matrix metalloproteinase-2 (MMP-2), membranous type 1 matrix metalloproteinase (MT1-MMP), and tissue inhibitor of metalloproteinase-2 (TIMP-2) in ectopic and eutopic endometrium from women with and without endometriosis throughout the menstrual cycle. Design: Molecular studies in human tissue. Setting: Reproductive immunology laboratory of a university medical center. Patient(s): Fifty-three premenopausal woman (23 with endometriosis and 30 without endometriosis) undergoing laparoscopic surgery. Endometrium and ectopic endometriosis tissue were obtained at the time of surgery. Main Outcome Measure(s): Messenger RNA and protein expression from eutopic and ectopic endometrium was analyzed by using quantitative competitive polymerase chain reaction, zymography, and Western blot assay. Result(s): Uterine endometrium from women with endometriosis expressed higher levels of MMP-2 and MT1-MMP and lower levels of TIMP-2 than did endometrium from normal women. Conclusion(s): Eutopic endometrium from patients with endometriosis may be more invasive and prone to peritoneal implantation because of greater expression of MMP-2 and MT1-MMP and lower expression of TIMP-2 messenger RNA, compared with endometrium from women without endometriosis. Thus, increased proteolytic activity may help to explain the invasive factors that result in endometriosis.

Original languageEnglish
Pages (from-to)787-795
Number of pages9
JournalFertility and Sterility
Volume78
Issue number4
DOIs
StatePublished - 1 Oct 2002

Keywords

  • Endometriosis
  • Matrix metalloproteinase-2
  • Membranous type 1 matrix metalloproteinase
  • Tissue inhibitor of metalloproteinase-2

Fingerprint

Dive into the research topics of 'Matrix metalloproteinase-2, membranous type 1 matrix metalloproteinase, and tissue inhibitor of metalloproteinase-2 expression in ectopic and eutopic endometrium'. Together they form a unique fingerprint.

Cite this