MARCKSL1 exhibits anti-angiogenic effects through suppression of VEGFR-2-dependent Akt/PDK-1/mTOR phosphorylation

Boh Ram Kim, Seung Hoon Lee, Mi Sun Park, Seung Hee Seo, Young Min Park, Young Joo Kwon, Seung Bae Rho

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Myristoylated alanine-rich C kinase substratelike 1 (MARCKSL1) plays a pivotal role in the regulation of apoptosis and has been shown to maintain antitumor and metastasis-suppressive properties. In the present study, we examined the effects of MARCKSL1 as a novel anti-angiogenic agent on the inhibition of angiogenesis-mediated cell migration. MARCKSL1 also reduced vascular endothelial growth factor (VEGF)-induced human umbilical vein endothelial cell (HUVEC) proliferation, as well as capillary-like tubular structure formation in vitro. MARCKSL1 disrupted phosphorylation of vascular endothelial growth factor receptor-2 (VEGFR-2) in ovarian tumorigenesis. In addition MARCKSL1 showed potent anti-angiogenic activity and reduced the levels of VEGF and hypoxia-inducible factor 1 (HIF-1) expression, an essential regulator of angiogenesis. Consistently, MARCKSL1 decreased VEGF induced phosphorylation of the PI3K/Akt signaling pathway components including phosphoinositide-dependent protein kinase 1 (PDK-1), mammalian target of rapamycin (mTOR), tuberous sclerosis complex 2 (TSC-2), p70 ribosomal protein S6 kinase (p70S6K), and glycogen synthase kinase 3 (GSK-3) protein. Collectively, our results provide evidence for the physiological/biological function of an endothelial cell system involved in angiogenesis through suppression of Akt/PDK-1/mTOR phosphorylation by interaction with VEGFR-2.

Original languageEnglish
Pages (from-to)1041-1048
Number of pages8
JournalOncology Reports
Volume35
Issue number2
DOIs
StatePublished - Feb 2016

Keywords

  • Akt/PDK-1/mTOR phosphorylation
  • Anti-angiogenic activity
  • Endothelial cells
  • MARCKSL1
  • Protein-protein interaction

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