Magneto-nanosensor platform for probing low-affinity protein-protein interaction: Characterization of PD-1 - PD-L1/PD-L2 inhibitory checkpoint axis

J. R. Lee; D. J. Bechstein; C. C. Ooi; A. Patel; R. S. Gaster; E. Ng; L. C. Gonzalez; S. X. Wang

Magneto-nanosensor platform for probing low-affinity protein-protein interaction: Characterization of PD-1 - PD-L1/PD-L2 inhibitory checkpoint axis

J. R. Lee
, D. J. Bechstein
, C. C. Ooi
, A. Patel
, R. S. Gaster
, E. Ng
, L. C. Gonzalez
, S. X. Wang

Department of Mechanical & Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Substantial efforts have been made to understand the interactions between immune checkpoint receptors and their ligands targeted in immunotherapies against cancer. To carefully characterize the complete network of interactions involved and the binding affinities between their extracellular domains, an improved kinetic assay is needed to overcome limitations with surface plasmon resonance (SPR). Here we present a magneto-nanosensor platform integrated with a microfluidic chip that allows measurement of dissociation constants in the micromolar-range. Using this platform, we characterized the binding affinities of the PD-1 - PD-L1/PD-L2 co-inhibitory receptor system, and discovered an unexpected interaction between PD-L1 and PD-L2.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	1176-1177
Number of pages	2
ISBN (Electronic)	9780979806490
State	Published - 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016

Publication series

Name	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	9/10/16 → 13/10/16

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Dissociation constants
Immune checkpoint receptors
Kinetic assays
Magnetic nanoparticles
Magneto-nanosensors
Microfluidics

Cite this

Lee, J. R., Bechstein, D. J., Ooi, C. C., Patel, A., Gaster, R. S., Ng, E., Gonzalez, L. C., & Wang, S. X. (2016). Magneto-nanosensor platform for probing low-affinity protein-protein interaction: Characterization of PD-1 - PD-L1/PD-L2 inhibitory checkpoint axis. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 1176-1177). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.

Lee, J. R. ; Bechstein, D. J. ; Ooi, C. C. et al. / Magneto-nanosensor platform for probing low-affinity protein-protein interaction : Characterization of PD-1 - PD-L1/PD-L2 inhibitory checkpoint axis. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 1176-1177 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

@inproceedings{f412ffc519ef4cb7aa029aacafa103f7,

title = "Magneto-nanosensor platform for probing low-affinity protein-protein interaction: Characterization of PD-1 - PD-L1/PD-L2 inhibitory checkpoint axis",

abstract = "Substantial efforts have been made to understand the interactions between immune checkpoint receptors and their ligands targeted in immunotherapies against cancer. To carefully characterize the complete network of interactions involved and the binding affinities between their extracellular domains, an improved kinetic assay is needed to overcome limitations with surface plasmon resonance (SPR). Here we present a magneto-nanosensor platform integrated with a microfluidic chip that allows measurement of dissociation constants in the micromolar-range. Using this platform, we characterized the binding affinities of the PD-1 - PD-L1/PD-L2 co-inhibitory receptor system, and discovered an unexpected interaction between PD-L1 and PD-L2.",

keywords = "Dissociation constants, Immune checkpoint receptors, Kinetic assays, Magnetic nanoparticles, Magneto-nanosensors, Microfluidics",

author = "Lee, \{J. R.\} and Bechstein, \{D. J.\} and Ooi, \{C. C.\} and A. Patel and Gaster, \{R. S.\} and E. Ng and Gonzalez, \{L. C.\} and Wang, \{S. X.\}",

year = "2016",

language = "English",

series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

publisher = "Chemical and Biological Microsystems Society",

pages = "1176--1177",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

}

Lee, JR, Bechstein, DJ, Ooi, CC, Patel, A, Gaster, RS, Ng, E, Gonzalez, LC & Wang, SX 2016, Magneto-nanosensor platform for probing low-affinity protein-protein interaction: Characterization of PD-1 - PD-L1/PD-L2 inhibitory checkpoint axis. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 1176-1177, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 9/10/16.

Magneto-nanosensor platform for probing low-affinity protein-protein interaction: Characterization of PD-1 - PD-L1/PD-L2 inhibitory checkpoint axis. / Lee, J. R.; Bechstein, D. J.; Ooi, C. C. et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 1176-1177 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Magneto-nanosensor platform for probing low-affinity protein-protein interaction

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

AU - Lee, J. R.

AU - Bechstein, D. J.

AU - Ooi, C. C.

AU - Patel, A.

AU - Gaster, R. S.

AU - Ng, E.

AU - Gonzalez, L. C.

AU - Wang, S. X.

PY - 2016

Y1 - 2016

N2 - Substantial efforts have been made to understand the interactions between immune checkpoint receptors and their ligands targeted in immunotherapies against cancer. To carefully characterize the complete network of interactions involved and the binding affinities between their extracellular domains, an improved kinetic assay is needed to overcome limitations with surface plasmon resonance (SPR). Here we present a magneto-nanosensor platform integrated with a microfluidic chip that allows measurement of dissociation constants in the micromolar-range. Using this platform, we characterized the binding affinities of the PD-1 - PD-L1/PD-L2 co-inhibitory receptor system, and discovered an unexpected interaction between PD-L1 and PD-L2.

AB - Substantial efforts have been made to understand the interactions between immune checkpoint receptors and their ligands targeted in immunotherapies against cancer. To carefully characterize the complete network of interactions involved and the binding affinities between their extracellular domains, an improved kinetic assay is needed to overcome limitations with surface plasmon resonance (SPR). Here we present a magneto-nanosensor platform integrated with a microfluidic chip that allows measurement of dissociation constants in the micromolar-range. Using this platform, we characterized the binding affinities of the PD-1 - PD-L1/PD-L2 co-inhibitory receptor system, and discovered an unexpected interaction between PD-L1 and PD-L2.

KW - Dissociation constants

KW - Immune checkpoint receptors

KW - Kinetic assays

KW - Magnetic nanoparticles

KW - Magneto-nanosensors

KW - Microfluidics

UR - https://www.scopus.com/pages/publications/85014291730

M3 - Conference contribution

AN - SCOPUS:85014291730

T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

SP - 1176

EP - 1177

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - Chemical and Biological Microsystems Society

Y2 - 9 October 2016 through 13 October 2016

ER -

Lee JR, Bechstein DJ, Ooi CC, Patel A, Gaster RS, Ng E et al. Magneto-nanosensor platform for probing low-affinity protein-protein interaction: Characterization of PD-1 - PD-L1/PD-L2 inhibitory checkpoint axis. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 1176-1177. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Magneto-nanosensor platform for probing low-affinity protein-protein interaction: Characterization of PD-1 - PD-L1/PD-L2 inhibitory checkpoint axis

Abstract

Publication series

Conference

UN SDGs

Keywords

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Cite this