@inproceedings{f412ffc519ef4cb7aa029aacafa103f7,
title = "Magneto-nanosensor platform for probing low-affinity protein-protein interaction: Characterization of PD-1 - PD-L1/PD-L2 inhibitory checkpoint axis",
abstract = "Substantial efforts have been made to understand the interactions between immune checkpoint receptors and their ligands targeted in immunotherapies against cancer. To carefully characterize the complete network of interactions involved and the binding affinities between their extracellular domains, an improved kinetic assay is needed to overcome limitations with surface plasmon resonance (SPR). Here we present a magneto-nanosensor platform integrated with a microfluidic chip that allows measurement of dissociation constants in the micromolar-range. Using this platform, we characterized the binding affinities of the PD-1 - PD-L1/PD-L2 co-inhibitory receptor system, and discovered an unexpected interaction between PD-L1 and PD-L2.",
keywords = "Dissociation constants, Immune checkpoint receptors, Kinetic assays, Magnetic nanoparticles, Magneto-nanosensors, Microfluidics",
author = "Lee, {J. R.} and Bechstein, {D. J.} and Ooi, {C. C.} and A. Patel and Gaster, {R. S.} and E. Ng and Gonzalez, {L. C.} and Wang, {S. X.}",
year = "2016",
language = "English",
series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
publisher = "Chemical and Biological Microsystems Society",
pages = "1176--1177",
booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",
}