Magneto-nanosensor platform for probing low-affinity protein-protein interaction: Characterization of PD-1 - PD-L1/PD-L2 inhibitory checkpoint axis

J. R. Lee, D. J. Bechstein, C. C. Ooi, A. Patel, R. S. Gaster, E. Ng, L. C. Gonzalez, S. X. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Substantial efforts have been made to understand the interactions between immune checkpoint receptors and their ligands targeted in immunotherapies against cancer. To carefully characterize the complete network of interactions involved and the binding affinities between their extracellular domains, an improved kinetic assay is needed to overcome limitations with surface plasmon resonance (SPR). Here we present a magneto-nanosensor platform integrated with a microfluidic chip that allows measurement of dissociation constants in the micromolar-range. Using this platform, we characterized the binding affinities of the PD-1 - PD-L1/PD-L2 co-inhibitory receptor system, and discovered an unexpected interaction between PD-L1 and PD-L2.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages1176-1177
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period9/10/1613/10/16

Keywords

  • Dissociation constants
  • Immune checkpoint receptors
  • Kinetic assays
  • Magnetic nanoparticles
  • Magneto-nanosensors
  • Microfluidics

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