Magnetic supercluster particles for highly sensitive magnetic biosensing of proteins

Songeun Kim; Junyoung Kim; Jisoo Im; Minah Kim; Taehyeong Kim; Shan X. Wang; Dokyoon Kim; Jung Rok Lee

doi:10.1007/s00604-022-05354-x

Magnetic supercluster particles for highly sensitive magnetic biosensing of proteins

Songeun Kim, Junyoung Kim, Jisoo Im, Minah Kim, Taehyeong Kim, Shan X. Wang, Dokyoon Kim, Jung Rok Lee

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A strategy is reported to improve the detection limits of current giant magnetoresistance (GMR) biosensors by augmenting the effective magnetic moment that the magnetic tags on the biosensors can exert. Magnetic supercluster particles (MSPs), each of which consists of ~ 1000 superparamagnetic cores, are prepared by a wet-chemical technique and are utilized to improve the limit of detection of GMR biosensors down to 17.6 zmol for biotin as a target molecule. This value is more than four orders of magnitude lower than that of the conventional colorimetric assay performed using the same set of reagents except for the signal transducer. The applicability of MSPs in immunoassay is further demonstrated by simultaneously detecting vascular endothelial growth factor (VEGF) and C-reactive protein (CRP) in a duplex assay format. MSPs outperform commercially available magnetic nanoparticles in terms of signal intensity and detection limit. Graphical abstract: [Figure not available: see fulltext.].

Original language	English
Article number	256
Journal	Microchimica Acta
Volume	189
Issue number	7
DOIs	https://doi.org/10.1007/s00604-022-05354-x
State	Published - Jul 2022

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2018R1A6A1A03024231, NRF-2018R1D1A1B07050979, NRF-2019M3C1B8090804, NRF-2020R1C1C1005416, NRF-2022R1A2C1003527). S.X.W. acknowledges support from the National Institutes of Health (NIH) grant R01CA25784301.

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.

Keywords

Biosensors
Giant magnetoresistance
Immunoassay
Magnetic nanoparticles
Supercluster particles

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10.1007/s00604-022-05354-x

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title = "Magnetic supercluster particles for highly sensitive magnetic biosensing of proteins",

abstract = "A strategy is reported to improve the detection limits of current giant magnetoresistance (GMR) biosensors by augmenting the effective magnetic moment that the magnetic tags on the biosensors can exert. Magnetic supercluster particles (MSPs), each of which consists of ~ 1000 superparamagnetic cores, are prepared by a wet-chemical technique and are utilized to improve the limit of detection of GMR biosensors down to 17.6 zmol for biotin as a target molecule. This value is more than four orders of magnitude lower than that of the conventional colorimetric assay performed using the same set of reagents except for the signal transducer. The applicability of MSPs in immunoassay is further demonstrated by simultaneously detecting vascular endothelial growth factor (VEGF) and C-reactive protein (CRP) in a duplex assay format. MSPs outperform commercially available magnetic nanoparticles in terms of signal intensity and detection limit. Graphical abstract: [Figure not available: see fulltext.].",

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author = "Songeun Kim and Junyoung Kim and Jisoo Im and Minah Kim and Taehyeong Kim and Wang, {Shan X.} and Dokyoon Kim and Lee, {Jung Rok}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2018R1A6A1A03024231, NRF-2018R1D1A1B07050979, NRF-2019M3C1B8090804, NRF-2020R1C1C1005416, NRF-2022R1A2C1003527). S.X.W. acknowledges support from the National Institutes of Health (NIH) grant R01CA25784301. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.",

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AU - Kim, Taehyeong

AU - Wang, Shan X.

AU - Kim, Dokyoon

AU - Lee, Jung Rok

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2018R1A6A1A03024231, NRF-2018R1D1A1B07050979, NRF-2019M3C1B8090804, NRF-2020R1C1C1005416, NRF-2022R1A2C1003527). S.X.W. acknowledges support from the National Institutes of Health (NIH) grant R01CA25784301. Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.

PY - 2022/7

Y1 - 2022/7

N2 - A strategy is reported to improve the detection limits of current giant magnetoresistance (GMR) biosensors by augmenting the effective magnetic moment that the magnetic tags on the biosensors can exert. Magnetic supercluster particles (MSPs), each of which consists of ~ 1000 superparamagnetic cores, are prepared by a wet-chemical technique and are utilized to improve the limit of detection of GMR biosensors down to 17.6 zmol for biotin as a target molecule. This value is more than four orders of magnitude lower than that of the conventional colorimetric assay performed using the same set of reagents except for the signal transducer. The applicability of MSPs in immunoassay is further demonstrated by simultaneously detecting vascular endothelial growth factor (VEGF) and C-reactive protein (CRP) in a duplex assay format. MSPs outperform commercially available magnetic nanoparticles in terms of signal intensity and detection limit. Graphical abstract: [Figure not available: see fulltext.].

AB - A strategy is reported to improve the detection limits of current giant magnetoresistance (GMR) biosensors by augmenting the effective magnetic moment that the magnetic tags on the biosensors can exert. Magnetic supercluster particles (MSPs), each of which consists of ~ 1000 superparamagnetic cores, are prepared by a wet-chemical technique and are utilized to improve the limit of detection of GMR biosensors down to 17.6 zmol for biotin as a target molecule. This value is more than four orders of magnitude lower than that of the conventional colorimetric assay performed using the same set of reagents except for the signal transducer. The applicability of MSPs in immunoassay is further demonstrated by simultaneously detecting vascular endothelial growth factor (VEGF) and C-reactive protein (CRP) in a duplex assay format. MSPs outperform commercially available magnetic nanoparticles in terms of signal intensity and detection limit. Graphical abstract: [Figure not available: see fulltext.].

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Magnetic supercluster particles for highly sensitive magnetic biosensing of proteins

Abstract

Bibliographical note

Keywords

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