TY - JOUR
T1 - Magnetic separation-enhanced photoluminescence detection of dipicolinic acid and quenching detection of Cu(II) ions
AU - Kim, Taehyeong
AU - Jeon, Hyeongjin
AU - Lee, Jung Rok
AU - Kim, Dokyoon
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2024/1/15
Y1 - 2024/1/15
N2 - Dipicolinic acid (DPA) is a chelate capable of binding to a variety of lanthanide ions to make them luminescent in the visible range. Based on this property and also assisted by magnetic separation, we report a strategy for the sensitive detection of DPA. Poly(acrylic acid)-coated iron oxide nanoparticles (IONPs) serve as a magnetic carrier to deliver only a necessary amount of Tb3+ ions to DPA in a sample solution. This enables photoluminescence measurement of the Tb3+-DPA complex with minimal background noise. The obtained detection limit, which is as low as 0.236 nM, is more than two orders of magnitude lower than that of the assay not assisted by magnetic separation. Not only does this method possess a potential for diagnosing anthrax, given that DPA is a major constituent of Bacillus anthracis spores, but it is also useful for detecting aqueous Cu2+ ions through the luminescence quenching effect. High sensitivity with a detection limit of 54 nM [Cu2+] is demonstrated using the Eu3+-DPA complex.
AB - Dipicolinic acid (DPA) is a chelate capable of binding to a variety of lanthanide ions to make them luminescent in the visible range. Based on this property and also assisted by magnetic separation, we report a strategy for the sensitive detection of DPA. Poly(acrylic acid)-coated iron oxide nanoparticles (IONPs) serve as a magnetic carrier to deliver only a necessary amount of Tb3+ ions to DPA in a sample solution. This enables photoluminescence measurement of the Tb3+-DPA complex with minimal background noise. The obtained detection limit, which is as low as 0.236 nM, is more than two orders of magnitude lower than that of the assay not assisted by magnetic separation. Not only does this method possess a potential for diagnosing anthrax, given that DPA is a major constituent of Bacillus anthracis spores, but it is also useful for detecting aqueous Cu2+ ions through the luminescence quenching effect. High sensitivity with a detection limit of 54 nM [Cu2+] is demonstrated using the Eu3+-DPA complex.
KW - Dipicolinic acid
KW - Iron oxide nanoparticle
KW - Lanthanide complex
KW - Photoluminescence quenching
KW - Photoluminscence assay
UR - http://www.scopus.com/inward/record.url?scp=85173815213&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2023.123501
DO - 10.1016/j.saa.2023.123501
M3 - Article
C2 - 37839210
AN - SCOPUS:85173815213
SN - 1386-1425
VL - 305
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
M1 - 123501
ER -