Lysobacter olei sp. nov., isolated from oil-contaminated soil

Dhiraj Kumar Chaudhary; Sang Don Lee; Jaisoo Kim

doi:10.1099/ijsem.0.002348

Lysobacter olei sp. nov., isolated from oil-contaminated soil

Dhiraj Kumar Chaudhary, Sang Don Lee, Jaisoo Kim

Department of Environmental Science & Engineering

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Strain D-14^T, a brown-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from oil-contaminated soil. It was able to grow at 20–40 °C, at pH 6.0–10.0 and at 0–1% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain D-14^T belonged to the genus Lysobacter and was closely related to Lysobacter caeni BUT-8^T (99.0% sequence similarity), Lysobacter ruishenii CTN-1^T (98.5%), Lysobacter daejeonensis GH1-9^T (98.2%) and Lysobacter panacisoli CJ29^T (97.2%). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl-N-methylethanolamine. The predominant fatty acids of strain D-14^T were iso-C_{15: 0}, iso-C_{16: 0}, summed feature 9 (iso-C_{17: 1} ω9c and/or C_{16: 0} 10-methyl), summed feature 3 (C_{16: 1} ω7c and/or C_{16: 1} ω6c), iso-C_{14: 0}, C_{11: 0}iso 3-OH, C_{15: 1}iso F and C_{16: 0}. The genomic DNA G+C content of this novel strain was 68.7 mol%. The DNA–DNA relatedness values between strain D-14^T and L. caeni BUT-8^T, L. ruishenii CTN-1^T, L. daejeonensis GH1-9^T and L. panacisoli CJ29^T were 56.0, 46.3, 48.7 and 41.7%, respectively, which fall below the threshold value of 70% for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain D-14^T represents a novel species of the genus Lysobacter, for which the name Lysobacterolei sp. nov. is proposed. The type strain is D-14^T (=KEMB 9005-572^T=KACC 19173^T=JCM 31917^T).

Original language	English
Pages (from-to)	4660-4666
Number of pages	7
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	67
Issue number	11
DOIs	https://doi.org/10.1099/ijsem.0.002348
State	Published - Nov 2017

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1A09916982).

Publisher Copyright:
© 2017 IUMS.

Keywords

Lysobacter olei sp. nov
Lysobacteraceae
Oil-contaminated soil
Proteobacteria

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10.1099/ijsem.0.002348

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@article{968c25e00a0e4080877fe90b6bf6fe51,

title = "Lysobacter olei sp. nov., isolated from oil-contaminated soil",

abstract = "Strain D-14T, a brown-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from oil-contaminated soil. It was able to grow at 20–40 °C, at pH 6.0–10.0 and at 0–1% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain D-14T belonged to the genus Lysobacter and was closely related to Lysobacter caeni BUT-8T (99.0% sequence similarity), Lysobacter ruishenii CTN-1T (98.5%), Lysobacter daejeonensis GH1-9T (98.2%) and Lysobacter panacisoli CJ29T (97.2%). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl-N-methylethanolamine. The predominant fatty acids of strain D-14T were iso-C15: 0, iso-C16: 0, summed feature 9 (iso-C17: 1 ω9c and/or C16: 0 10-methyl), summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), iso-C14: 0, C11: 0iso 3-OH, C15: 1iso F and C16: 0. The genomic DNA G+C content of this novel strain was 68.7 mol%. The DNA–DNA relatedness values between strain D-14T and L. caeni BUT-8T, L. ruishenii CTN-1T, L. daejeonensis GH1-9T and L. panacisoli CJ29T were 56.0, 46.3, 48.7 and 41.7%, respectively, which fall below the threshold value of 70% for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain D-14T represents a novel species of the genus Lysobacter, for which the name Lysobacterolei sp. nov. is proposed. The type strain is D-14T (=KEMB 9005-572T=KACC 19173T=JCM 31917T).",

keywords = "Lysobacter olei sp. nov, Lysobacteraceae, Oil-contaminated soil, Proteobacteria",

author = "Chaudhary, {Dhiraj Kumar} and Lee, {Sang Don} and Jaisoo Kim",

note = "Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1A09916982). Publisher Copyright: {\textcopyright} 2017 IUMS.",

year = "2017",

month = nov,

doi = "10.1099/ijsem.0.002348",

language = "English",

volume = "67",

pages = "4660--4666",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5026",

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T1 - Lysobacter olei sp. nov., isolated from oil-contaminated soil

AU - Chaudhary, Dhiraj Kumar

AU - Lee, Sang Don

AU - Kim, Jaisoo

N1 - Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1A09916982). Publisher Copyright: © 2017 IUMS.

PY - 2017/11

Y1 - 2017/11

N2 - Strain D-14T, a brown-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from oil-contaminated soil. It was able to grow at 20–40 °C, at pH 6.0–10.0 and at 0–1% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain D-14T belonged to the genus Lysobacter and was closely related to Lysobacter caeni BUT-8T (99.0% sequence similarity), Lysobacter ruishenii CTN-1T (98.5%), Lysobacter daejeonensis GH1-9T (98.2%) and Lysobacter panacisoli CJ29T (97.2%). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl-N-methylethanolamine. The predominant fatty acids of strain D-14T were iso-C15: 0, iso-C16: 0, summed feature 9 (iso-C17: 1 ω9c and/or C16: 0 10-methyl), summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), iso-C14: 0, C11: 0iso 3-OH, C15: 1iso F and C16: 0. The genomic DNA G+C content of this novel strain was 68.7 mol%. The DNA–DNA relatedness values between strain D-14T and L. caeni BUT-8T, L. ruishenii CTN-1T, L. daejeonensis GH1-9T and L. panacisoli CJ29T were 56.0, 46.3, 48.7 and 41.7%, respectively, which fall below the threshold value of 70% for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain D-14T represents a novel species of the genus Lysobacter, for which the name Lysobacterolei sp. nov. is proposed. The type strain is D-14T (=KEMB 9005-572T=KACC 19173T=JCM 31917T).

AB - Strain D-14T, a brown-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from oil-contaminated soil. It was able to grow at 20–40 °C, at pH 6.0–10.0 and at 0–1% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain D-14T belonged to the genus Lysobacter and was closely related to Lysobacter caeni BUT-8T (99.0% sequence similarity), Lysobacter ruishenii CTN-1T (98.5%), Lysobacter daejeonensis GH1-9T (98.2%) and Lysobacter panacisoli CJ29T (97.2%). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl-N-methylethanolamine. The predominant fatty acids of strain D-14T were iso-C15: 0, iso-C16: 0, summed feature 9 (iso-C17: 1 ω9c and/or C16: 0 10-methyl), summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), iso-C14: 0, C11: 0iso 3-OH, C15: 1iso F and C16: 0. The genomic DNA G+C content of this novel strain was 68.7 mol%. The DNA–DNA relatedness values between strain D-14T and L. caeni BUT-8T, L. ruishenii CTN-1T, L. daejeonensis GH1-9T and L. panacisoli CJ29T were 56.0, 46.3, 48.7 and 41.7%, respectively, which fall below the threshold value of 70% for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain D-14T represents a novel species of the genus Lysobacter, for which the name Lysobacterolei sp. nov. is proposed. The type strain is D-14T (=KEMB 9005-572T=KACC 19173T=JCM 31917T).

KW - Lysobacter olei sp. nov

KW - Lysobacteraceae

KW - Oil-contaminated soil

KW - Proteobacteria

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ER -

Lysobacter olei sp. nov., isolated from oil-contaminated soil

Abstract

Bibliographical note

Keywords

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