Lysobacter olei sp. nov., isolated from oil-contaminated soil

Strain D-14^T, a brown-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from oil-contaminated soil. It was able to grow at 20–40 °C, at pH 6.0–10.0 and at 0–1% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain D-14^T belonged to the genus Lysobacter and was closely related to Lysobacter caeni BUT-8^T (99.0% sequence similarity), Lysobacter ruishenii CTN-1^T (98.5%), Lysobacter daejeonensis GH1-9^T (98.2%) and Lysobacter panacisoli CJ29^T (97.2%). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl-N-methylethanolamine. The predominant fatty acids of strain D-14^T were iso-C_{15: 0}, iso-C_{16: 0}, summed feature 9 (iso-C_{17: 1} ω9c and/or C_{16: 0} 10-methyl), summed feature 3 (C_{16: 1} ω7c and/or C_{16: 1} ω6c), iso-C_{14: 0}, C_{11: 0}iso 3-OH, C_{15: 1}iso F and C_{16: 0}. The genomic DNA G+C content of this novel strain was 68.7 mol%. The DNA–DNA relatedness values between strain D-14^T and L. caeni BUT-8^T, L. ruishenii CTN-1^T, L. daejeonensis GH1-9^T and L. panacisoli CJ29^T were 56.0, 46.3, 48.7 and 41.7%, respectively, which fall below the threshold value of 70% for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain D-14^T represents a novel species of the genus Lysobacter, for which the name Lysobacterolei sp. nov. is proposed. The type strain is D-14^T (=KEMB 9005-572^T=KACC 19173^T=JCM 31917^T).