Lysates of a probiotic, lactobacillus rhamnosus, can improve skin barrier function in a reconstructed human epidermis model

Ye On Jung, Haengdueng Jeong, Yejin Cho, Eun Ok Lee, Hye Won Jang, Jinwook Kim, Ki Taek Nam, Kyung Min Lim

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


The main function of the skin is to protect the body from the external environment. The barrier function of the skin is mainly provided by the stratum corneum, which consists of corneocytes bound with the corneodesmosomes and lamellar lipids. Skin barrier proteins like loricrin and filaggrin also contribute to the skin barrier function. In various skin diseases, skin barrier dysfunction is a common symptom, and skin irritants like detergents or surfactants could also perturb skin barrier function. Many efforts have been made to develop strategies to improve skin barrier function. Here, we investigated whether the microfluidized lysates of Lactobacillus rhamnosus (LR), one of the most widely used probiotic species for various health benefits, may improve the skin barrier function in a reconstructed human epidermis, Keraskin™. Application of LR lysate on Keraskin™ increased the expression of tight junction proteins; claudin 1 and occludin as determined by immunofluorescence analysis, and skin barrier proteins; loricrin and filaggrin as determined by immunohistochemistry and immunofluorescence analysis and qPCR. Also, the cytotoxicity of a skin irritant, sodium lauryl sulfate (SLS), was alleviated by the pretreatment of LR lysate. The skin barrier protective effects of LR lysate could be further demonstrated by the attenuation of SLS-enhanced dye-penetration. LR lysate also attenuated the destruction of desmosomes after SLS treatment. Collectively, we demonstrated that LR lysate has protective effects on the skin barrier, which could expand the utility of probiotics to skin-moisturization ingredients.

Original languageEnglish
Article number4289
JournalInternational Journal of Molecular Sciences
Issue number17
StatePublished - 1 Sep 2019


  • Lactobacillus rhamnosus
  • Probiotic
  • Reconstructed human epidermis model
  • Skin
  • Skin barrier


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