Low-Affinity Zinc Sensor Showing Fluorescence Responses with Minimal Artifacts

Xinhao Yan; Jin Ju Kim; Hye Sun Jeong; Yu Kyung Moon; Yoon Kyung Cho; Soyeon Ahn; Sang Beom Jun; Hakwon Kim; Youngmin You

doi:10.1021/acs.inorgchem.6b02786

Low-Affinity Zinc Sensor Showing Fluorescence Responses with Minimal Artifacts

Xinhao Yan, Jin Ju Kim, Hye Sun Jeong, Yu Kyung Moon, Yoon Kyung Cho, Soyeon Ahn, Sang Beom Jun, Hakwon Kim, Youngmin You

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The study of the zinc biology requires molecular probes with proper zinc affinity. We developed a low-affinity zinc probe (HBO-ACR) based on an azacrown ether (ACR) and an 2-(2-hydroxyphenyl)benzoxazole (HBO) fluorophore. This probe design imposed positive charge in the vicinity of a zinc coordination center, which enabled fluorescence turn-on responses to high levels of zinc without being affected by the pH and the presence of other transition-metal ions. Steady-state and transient photophysical investigations suggested that such a high tolerance benefits from orchestrated actions of proton-induced nonradiative and zinc-induced radiative control. The zinc bioimaging utility of HBO-ACR has been fully demonstrated with the use of human pancreas epidermoid carcinoma, PANC-1 cells, and rodent hippocampal neurons from cultures and acute brain slices. The results obtained through our studies established the validity of incorporating positively charged ionophores for the creation of low-affinity probes for the visualization of biometals.

Original language	English
Pages (from-to)	4332-4346
Number of pages	15
Journal	Inorganic Chemistry
Volume	56
Issue number	8
DOIs	https://doi.org/10.1021/acs.inorgchem.6b02786
State	Published - 17 Apr 2017

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© 2017 American Chemical Society.

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title = "Low-Affinity Zinc Sensor Showing Fluorescence Responses with Minimal Artifacts",

abstract = "The study of the zinc biology requires molecular probes with proper zinc affinity. We developed a low-affinity zinc probe (HBO-ACR) based on an azacrown ether (ACR) and an 2-(2-hydroxyphenyl)benzoxazole (HBO) fluorophore. This probe design imposed positive charge in the vicinity of a zinc coordination center, which enabled fluorescence turn-on responses to high levels of zinc without being affected by the pH and the presence of other transition-metal ions. Steady-state and transient photophysical investigations suggested that such a high tolerance benefits from orchestrated actions of proton-induced nonradiative and zinc-induced radiative control. The zinc bioimaging utility of HBO-ACR has been fully demonstrated with the use of human pancreas epidermoid carcinoma, PANC-1 cells, and rodent hippocampal neurons from cultures and acute brain slices. The results obtained through our studies established the validity of incorporating positively charged ionophores for the creation of low-affinity probes for the visualization of biometals.",

author = "Xinhao Yan and Kim, {Jin Ju} and Jeong, {Hye Sun} and Moon, {Yu Kyung} and Cho, {Yoon Kyung} and Soyeon Ahn and Jun, {Sang Beom} and Hakwon Kim and Youngmin You",

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AU - Yan, Xinhao

AU - Kim, Jin Ju

AU - Jeong, Hye Sun

AU - Moon, Yu Kyung

AU - Cho, Yoon Kyung

AU - Ahn, Soyeon

AU - Jun, Sang Beom

AU - Kim, Hakwon

AU - You, Youngmin

PY - 2017/4/17

Y1 - 2017/4/17

N2 - The study of the zinc biology requires molecular probes with proper zinc affinity. We developed a low-affinity zinc probe (HBO-ACR) based on an azacrown ether (ACR) and an 2-(2-hydroxyphenyl)benzoxazole (HBO) fluorophore. This probe design imposed positive charge in the vicinity of a zinc coordination center, which enabled fluorescence turn-on responses to high levels of zinc without being affected by the pH and the presence of other transition-metal ions. Steady-state and transient photophysical investigations suggested that such a high tolerance benefits from orchestrated actions of proton-induced nonradiative and zinc-induced radiative control. The zinc bioimaging utility of HBO-ACR has been fully demonstrated with the use of human pancreas epidermoid carcinoma, PANC-1 cells, and rodent hippocampal neurons from cultures and acute brain slices. The results obtained through our studies established the validity of incorporating positively charged ionophores for the creation of low-affinity probes for the visualization of biometals.

AB - The study of the zinc biology requires molecular probes with proper zinc affinity. We developed a low-affinity zinc probe (HBO-ACR) based on an azacrown ether (ACR) and an 2-(2-hydroxyphenyl)benzoxazole (HBO) fluorophore. This probe design imposed positive charge in the vicinity of a zinc coordination center, which enabled fluorescence turn-on responses to high levels of zinc without being affected by the pH and the presence of other transition-metal ions. Steady-state and transient photophysical investigations suggested that such a high tolerance benefits from orchestrated actions of proton-induced nonradiative and zinc-induced radiative control. The zinc bioimaging utility of HBO-ACR has been fully demonstrated with the use of human pancreas epidermoid carcinoma, PANC-1 cells, and rodent hippocampal neurons from cultures and acute brain slices. The results obtained through our studies established the validity of incorporating positively charged ionophores for the creation of low-affinity probes for the visualization of biometals.

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SN - 0020-1669

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EP - 4346

JO - Inorganic Chemistry

JF - Inorganic Chemistry

IS - 8

ER -

Low-Affinity Zinc Sensor Showing Fluorescence Responses with Minimal Artifacts

Abstract

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