Long wavelength TCF-based fluorescence probe for the detection of Alkaline Phosphatase in live cells

Lauren Gwynne; Adam C. Sedgwick; Jordan E. Gardiner; George T. Williams; Gyoungmi Kim; John P. Lowe; Jean Yves Maillard; Toby Jenkins; Steven D. Bull; Jonathan L. Sessler; Juyoung Yoon; Tony D. James

doi:10.3389/fchem.2019.00255

Long wavelength TCF-based fluorescence probe for the detection of Alkaline Phosphatase in live cells

Lauren Gwynne
, Adam C. Sedgwick
, Jordan E. Gardiner
, George T. Williams
, Gyoungmi Kim
, John P. Lowe
, Jean Yves Maillard
, Toby Jenkins
, Steven D. Bull
, Jonathan L. Sessler
, Juyoung Yoon
, Tony D. James

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

A long wavelength TCF-based fluorescence probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence 'turn on' (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.

Original language	English
Article number	255
Journal	Frontiers in Chemistry
Volume	7
Issue number	APR
DOIs	https://doi.org/10.3389/fchem.2019.00255
State	Published - 2019

Bibliographical note

Publisher Copyright:
© 2019 Gwynne, Sedgwick, Gardiner, Williams, Kim, Maillard, Jenkins, Bull, Sessler, Yoon and James.

Keywords

Alkaline Phosphatase
Cell imaging
Colorimetric
Fluorescence
Reaction-based fluorescent probe

Access to Document

10.3389/fchem.2019.00255

Cite this

@article{080a9f2c9b9843889704c045db3febed,

title = "Long wavelength TCF-based fluorescence probe for the detection of Alkaline Phosphatase in live cells",

abstract = "A long wavelength TCF-based fluorescence probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence 'turn on' (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.",

keywords = "Alkaline Phosphatase, Cell imaging, Colorimetric, Fluorescence, Reaction-based fluorescent probe",

author = "Lauren Gwynne and Sedgwick, \{Adam C.\} and Gardiner, \{Jordan E.\} and Williams, \{George T.\} and Gyoungmi Kim and Lowe, \{John P.\} and Maillard, \{Jean Yves\} and Toby Jenkins and Bull, \{Steven D.\} and Sessler, \{Jonathan L.\} and Juyoung Yoon and James, \{Tony D.\}",

note = "Publisher Copyright: {\textcopyright} 2019 Gwynne, Sedgwick, Gardiner, Williams, Kim, Maillard, Jenkins, Bull, Sessler, Yoon and James.",

year = "2019",

doi = "10.3389/fchem.2019.00255",

language = "English",

volume = "7",

journal = "Frontiers in Chemistry",

issn = "2296-2646",

publisher = "Frontiers Media S.A.",

number = "APR",

}

TY - JOUR

T1 - Long wavelength TCF-based fluorescence probe for the detection of Alkaline Phosphatase in live cells

AU - Gwynne, Lauren

AU - Sedgwick, Adam C.

AU - Gardiner, Jordan E.

AU - Williams, George T.

AU - Kim, Gyoungmi

AU - Lowe, John P.

AU - Maillard, Jean Yves

AU - Jenkins, Toby

AU - Bull, Steven D.

AU - Sessler, Jonathan L.

AU - Yoon, Juyoung

AU - James, Tony D.

PY - 2019

Y1 - 2019

N2 - A long wavelength TCF-based fluorescence probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence 'turn on' (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.

AB - A long wavelength TCF-based fluorescence probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence 'turn on' (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.

KW - Alkaline Phosphatase

KW - Cell imaging

KW - Colorimetric

KW - Fluorescence

KW - Reaction-based fluorescent probe

UR - https://www.scopus.com/pages/publications/85064659516

U2 - 10.3389/fchem.2019.00255

DO - 10.3389/fchem.2019.00255

M3 - Article

AN - SCOPUS:85064659516

SN - 2296-2646

VL - 7

JO - Frontiers in Chemistry

JF - Frontiers in Chemistry

IS - APR

M1 - 255

ER -

Long wavelength TCF-based fluorescence probe for the detection of Alkaline Phosphatase in live cells

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this