To Investigate starvation effect on methanogen community, two Identical membrane reactors were continuously operated for 84 consecutive days, with a temperature change from 50°C to 20°C. Continuous feeding washed out 97% biomass from reactors during the experimental period. Quantitative PCR, using mcrA genes, indicated that the methanogen abundance decreased from 7.0×107 to 1.2×107 mcrA copies·ml-1 (volume basis) at 50 °C, and then increased to 4.4 ×107 mcrA coples-ml-1 at 20 °C (p<0.05). Correspondence analysis Indicated that methanogen communities were distinctly grouped by each temperature. Canonical correspondence analysis Indicated that temperature showed a significant correlation with the methanogen community composition. These results suggest that methanogens can survive for a long time (at least more than 84 days) under starvation conditions, and that temperature could be a primary factor determining the density and community of methanogens.
|Number of pages||5|
|Journal||Journal of Environmental Biology|
|State||Published - 1 Mar 2015|
- Population dynamics