Long-term monitoring of yeast cell division via elastic micro-pad

Sung S. Lee, Ima Avalos Vizcarra, Luke P. Lee, Matthias Heinemann

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Microscopic long-term monitoring of yeast cell division is essential to study agerelated diseases. However, the exponential increase in the number of offspring makes it impossible at some point to track the original mother cell. We overcame this current limitation with utilizing a microfluidic platform. The height of micro-pad was designed as a similar dimension of a yeast cell in order to hold mother cell. By providing media flow, budded cell was removed, and the division event of mother cell beneath micro-pad was continuously monitored in single cell level. This platform could be applied to study yeast under dynamically metabolic environments.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages478-480
Number of pages3
ISBN (Print)9780979806421
StatePublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 1 Nov 20095 Nov 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/TerritoryKorea, Republic of
CityJeju
Period1/11/095/11/09

Keywords

  • Long-term monitoring
  • Metabolism
  • Single cell analysis
  • Yeast cell division

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