Long-term cytotoxic drug assay via single-cell microfluidic array

Liz Y. Wu; Dino Di Carlo; Luke P. Lee

Long-term cytotoxic drug assay via single-cell microfluidic array

Liz Y. Wu, Dino Di Carlo, Luke P. Lee

Department of Chemistry & Nanoscience

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

In this work, we use a single cell trapping array device for cytotoxic drug investigations on a large amount of single cells. The uniform and precise microenvironment for each single cell rigorously controls the drug assay experiment. The dynamic perfusion flow supplies media with/without drug to properly simulate the in vivo drug delivery mode. Drug assays for both adherent and non-adherent cell lines were demonstrated in the device. We have evaluated dose conditions of Taxol on HeLa and HL-60 cells. By tracing each single cells before, during and after the drug exposure, we first correlate the lifetime of the cells and their condition when exposing to the drug.

Original language	English
Title of host publication	Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Editors	Jean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
Publisher	Chemical and Biological Microsystems Society
Pages	709-711
Number of pages	3
ISBN (Electronic)	9780979806407
State	Published - 2007
Event	11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France Duration: 7 Oct 2007 → 11 Oct 2007

Publication series

Name	Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference	11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Country/Territory	France
City	Paris
Period	7/10/07 → 11/10/07

Bibliographical note

Funding Information:
This research was supported by Intel Research Fund, GSK, Taiwan Merit Scholarship TMS-094-2-A-008 (L.W.)

Funding Information:
This research was supported by Intel eR search Fund, GSK, Taiwan Merit Scholarship TMS-094-2-A-008 (L.W.)

Publisher Copyright:
© 2007 CBMS.

Keywords

Cell culture
Drug assay
Microfluidic devices
Single cell analysis

Cite this

Wu, L. Y., Di Carlo, D., & Lee, L. P. (2007). Long-term cytotoxic drug assay via single-cell microfluidic array. In J.-L. Viovy, P. Tabeling, S. Descroix, & L. Malaquin (Eds.), Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 (pp. 709-711). (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007). Chemical and Biological Microsystems Society.

Wu, Liz Y. ; Di Carlo, Dino ; Lee, Luke P. / Long-term cytotoxic drug assay via single-cell microfluidic array. Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. editor / Jean-Louis Viovy ; Patrick Tabeling ; Stephanie Descroix ; Laurent Malaquin. Chemical and Biological Microsystems Society, 2007. pp. 709-711 (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007).

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abstract = "In this work, we use a single cell trapping array device for cytotoxic drug investigations on a large amount of single cells. The uniform and precise microenvironment for each single cell rigorously controls the drug assay experiment. The dynamic perfusion flow supplies media with/without drug to properly simulate the in vivo drug delivery mode. Drug assays for both adherent and non-adherent cell lines were demonstrated in the device. We have evaluated dose conditions of Taxol on HeLa and HL-60 cells. By tracing each single cells before, during and after the drug exposure, we first correlate the lifetime of the cells and their condition when exposing to the drug.",

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note = "Funding Information: This research was supported by Intel Research Fund, GSK, Taiwan Merit Scholarship TMS-094-2-A-008 (L.W.) Funding Information: This research was supported by Intel eR search Fund, GSK, Taiwan Merit Scholarship TMS-094-2-A-008 (L.W.) Publisher Copyright: {\textcopyright} 2007 CBMS.; 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 ; Conference date: 07-10-2007 Through 11-10-2007",

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publisher = "Chemical and Biological Microsystems Society",

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Wu, LY, Di Carlo, D & Lee, LP 2007, Long-term cytotoxic drug assay via single-cell microfluidic array. in J-L Viovy, P Tabeling, S Descroix & L Malaquin (eds), Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007, Chemical and Biological Microsystems Society, pp. 709-711, 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007, Paris, France, 7/10/07.

Long-term cytotoxic drug assay via single-cell microfluidic array. / Wu, Liz Y.; Di Carlo, Dino; Lee, Luke P.
Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. ed. / Jean-Louis Viovy; Patrick Tabeling; Stephanie Descroix; Laurent Malaquin. Chemical and Biological Microsystems Society, 2007. p. 709-711 (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Di Carlo, Dino

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N1 - Funding Information: This research was supported by Intel Research Fund, GSK, Taiwan Merit Scholarship TMS-094-2-A-008 (L.W.) Funding Information: This research was supported by Intel eR search Fund, GSK, Taiwan Merit Scholarship TMS-094-2-A-008 (L.W.) Publisher Copyright: © 2007 CBMS.

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N2 - In this work, we use a single cell trapping array device for cytotoxic drug investigations on a large amount of single cells. The uniform and precise microenvironment for each single cell rigorously controls the drug assay experiment. The dynamic perfusion flow supplies media with/without drug to properly simulate the in vivo drug delivery mode. Drug assays for both adherent and non-adherent cell lines were demonstrated in the device. We have evaluated dose conditions of Taxol on HeLa and HL-60 cells. By tracing each single cells before, during and after the drug exposure, we first correlate the lifetime of the cells and their condition when exposing to the drug.

AB - In this work, we use a single cell trapping array device for cytotoxic drug investigations on a large amount of single cells. The uniform and precise microenvironment for each single cell rigorously controls the drug assay experiment. The dynamic perfusion flow supplies media with/without drug to properly simulate the in vivo drug delivery mode. Drug assays for both adherent and non-adherent cell lines were demonstrated in the device. We have evaluated dose conditions of Taxol on HeLa and HL-60 cells. By tracing each single cells before, during and after the drug exposure, we first correlate the lifetime of the cells and their condition when exposing to the drug.

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Wu LY, Di Carlo D, Lee LP. Long-term cytotoxic drug assay via single-cell microfluidic array. In Viovy JL, Tabeling P, Descroix S, Malaquin L, editors, Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. Chemical and Biological Microsystems Society. 2007. p. 709-711. (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007).

Long-term cytotoxic drug assay via single-cell microfluidic array

Abstract

Publication series

Conference

Bibliographical note

Keywords

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Cite this