Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets

Kenichi Nagase, Yuhei Nagumo, Miri Kim, Hee Jung Kim, Hei Won Kyung, Hye Jin Chung, Hidekazu Sekine, Tatsuya Shimizu, Hideko Kanazawa, Teruo Okano, Seung Jin Lee, Masayuki Yamato

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47 Scopus citations


Cell sheet transplantation is a key tissue engineering technology. A vascular endothelial growth factor (VEGF)-releasing fiber mat is developed for the transplantation of multilayered cardiomyocyte sheets. Poly(vinyl alcohol) fiber mats bearing poly(lactic-co-glycolic acid) nanoparticles that incorporate VEGF are fabricated using electrospinning and electrospray methods. Six-layered cardiomyocyte sheets are transplanted with a VEGF-releasing mat into athymic rats. After two weeks, these sheets produce thicker cardiomyocyte layers compared with controls lacking a VEGF-releasing mat, and incorporate larger-diameter blood vessels containing erythrocytes. Thus, local VEGF release near the transplanted cardiomyocytes induces vascularization, which supplies sufficient oxygen and nutrients to prevent necrosis. In contrast, cardiomyocyte sheets without a VEGF-releasing mat do not survive in vivo, probably undergo necrosis, and are reduced in thickness. Hence, these VEGF-releasing mats enable the transplantation of multilayered cardiomyocyte sheets in a single procedure, and should expand the potential of cell sheet transplantation for therapeutic applications. (Figure presented.).

Original languageEnglish
Article number1700073
JournalMacromolecular Bioscience
Issue number8
StatePublished - Aug 2017

Bibliographical note

Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim


  • VEGF
  • cell sheet
  • electrospun fiber
  • regenerative medicine
  • tissue engineering


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