Lipid-Soluble Ginseng Extract Induces Apoptosis and G0/G1 Cell Cycle Arrest in NCI-H460 Human Lung Cancer Cells

Moo Rim Kang; Hwan Mook Kim; Jong Soon Kang; Kiho Lee; Sung Dong Lee; Dong Hoon Hyun; Man Jin In; Song Kyu Park; Dong Chung Kim

doi:10.1007/s11130-011-0232-6

Lipid-Soluble Ginseng Extract Induces Apoptosis and G0/G1 Cell Cycle Arrest in NCI-H460 Human Lung Cancer Cells

Moo Rim Kang
, Hwan Mook Kim
, Jong Soon Kang
, Kiho Lee
, Sung Dong Lee
, Dong Hoon Hyun
, Man Jin In
, Song Kyu Park
, Dong Chung Kim

Department of Life Science

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

This study was performed to elucidate the anticancer mechanism of a lipid-soluble ginseng extract (LSGE) by analyzing induction of apoptosis and arrest of cell cycle progression using the NCI-H460 human lung cancer cell line. Proliferation of NCI-H460 cells was potently inhibited by LSGE in a dose-dependent manner. The cell cycle arrest at the G0/G1 phase in NCI-H460 cells was induced by LSGE. The percentage of G0/G1 phase cells significantly increased, while that of S phase cells decreased after treatment with LSGE. The expr.occurred through apoptosis, which was accompanied by increasing the activity of caspases including caspase-8, caspase-9 and caspase-3. Consistent with enhancement of caspase activity, LSGE increased protein levels of cleaved caspase-3, caspase-8, caspase-9, and poly-ADP-ribose polymerase (PARP). These apoptotic effects of LSGE were inhibited by the pan-caspase inhibitor Z-VAD-fmk. These findings indicate that LSGE inhibits NCI-H460 human lung cancer cell growth by cell cycle arrest at the G0/G1 phase and induction of caspase-mediated apoptosis.

Original language	English
Pages (from-to)	101-106
Number of pages	6
Journal	Plant Foods for Human Nutrition
Volume	66
Issue number	2
DOIs	https://doi.org/10.1007/s11130-011-0232-6
State	Published - Jun 2011

Bibliographical note

Funding Information:
Acknowledgements This study was supported in part by a grant (No. A101836) of the Korean Health Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea and a grant from the KRIBB Research Initiative Program.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptosis induction
Caspase activation
G0/G1 phase arrest
Human lung cancer cell
Lipid-soluble ginseng extract

Access to Document

10.1007/s11130-011-0232-6

Cite this

@article{54345404a0354cacb10bc4477f9babf1,

title = "Lipid-Soluble Ginseng Extract Induces Apoptosis and G0/G1 Cell Cycle Arrest in NCI-H460 Human Lung Cancer Cells",

abstract = "This study was performed to elucidate the anticancer mechanism of a lipid-soluble ginseng extract (LSGE) by analyzing induction of apoptosis and arrest of cell cycle progression using the NCI-H460 human lung cancer cell line. Proliferation of NCI-H460 cells was potently inhibited by LSGE in a dose-dependent manner. The cell cycle arrest at the G0/G1 phase in NCI-H460 cells was induced by LSGE. The percentage of G0/G1 phase cells significantly increased, while that of S phase cells decreased after treatment with LSGE. The expr.occurred through apoptosis, which was accompanied by increasing the activity of caspases including caspase-8, caspase-9 and caspase-3. Consistent with enhancement of caspase activity, LSGE increased protein levels of cleaved caspase-3, caspase-8, caspase-9, and poly-ADP-ribose polymerase (PARP). These apoptotic effects of LSGE were inhibited by the pan-caspase inhibitor Z-VAD-fmk. These findings indicate that LSGE inhibits NCI-H460 human lung cancer cell growth by cell cycle arrest at the G0/G1 phase and induction of caspase-mediated apoptosis.",

keywords = "Apoptosis induction, Caspase activation, G0/G1 phase arrest, Human lung cancer cell, Lipid-soluble ginseng extract",

author = "Kang, \{Moo Rim\} and Kim, \{Hwan Mook\} and Kang, \{Jong Soon\} and Kiho Lee and Lee, \{Sung Dong\} and Hyun, \{Dong Hoon\} and In, \{Man Jin\} and Park, \{Song Kyu\} and Kim, \{Dong Chung\}",

note = "Funding Information: Acknowledgements This study was supported in part by a grant (No. A101836) of the Korean Health Technology R\&D Project, Ministry for Health, Welfare \& Family Affairs, Republic of Korea and a grant from the KRIBB Research Initiative Program.",

year = "2011",

month = jun,

doi = "10.1007/s11130-011-0232-6",

language = "English",

volume = "66",

pages = "101--106",

journal = "Plant Foods for Human Nutrition",

issn = "0921-9668",

publisher = "Springer",

number = "2",

}

TY - JOUR

T1 - Lipid-Soluble Ginseng Extract Induces Apoptosis and G0/G1 Cell Cycle Arrest in NCI-H460 Human Lung Cancer Cells

AU - Kang, Moo Rim

AU - Kim, Hwan Mook

AU - Kang, Jong Soon

AU - Lee, Kiho

AU - Lee, Sung Dong

AU - Hyun, Dong Hoon

AU - In, Man Jin

AU - Park, Song Kyu

AU - Kim, Dong Chung

N1 - Funding Information: Acknowledgements This study was supported in part by a grant (No. A101836) of the Korean Health Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea and a grant from the KRIBB Research Initiative Program.

PY - 2011/6

Y1 - 2011/6

N2 - This study was performed to elucidate the anticancer mechanism of a lipid-soluble ginseng extract (LSGE) by analyzing induction of apoptosis and arrest of cell cycle progression using the NCI-H460 human lung cancer cell line. Proliferation of NCI-H460 cells was potently inhibited by LSGE in a dose-dependent manner. The cell cycle arrest at the G0/G1 phase in NCI-H460 cells was induced by LSGE. The percentage of G0/G1 phase cells significantly increased, while that of S phase cells decreased after treatment with LSGE. The expr.occurred through apoptosis, which was accompanied by increasing the activity of caspases including caspase-8, caspase-9 and caspase-3. Consistent with enhancement of caspase activity, LSGE increased protein levels of cleaved caspase-3, caspase-8, caspase-9, and poly-ADP-ribose polymerase (PARP). These apoptotic effects of LSGE were inhibited by the pan-caspase inhibitor Z-VAD-fmk. These findings indicate that LSGE inhibits NCI-H460 human lung cancer cell growth by cell cycle arrest at the G0/G1 phase and induction of caspase-mediated apoptosis.

AB - This study was performed to elucidate the anticancer mechanism of a lipid-soluble ginseng extract (LSGE) by analyzing induction of apoptosis and arrest of cell cycle progression using the NCI-H460 human lung cancer cell line. Proliferation of NCI-H460 cells was potently inhibited by LSGE in a dose-dependent manner. The cell cycle arrest at the G0/G1 phase in NCI-H460 cells was induced by LSGE. The percentage of G0/G1 phase cells significantly increased, while that of S phase cells decreased after treatment with LSGE. The expr.occurred through apoptosis, which was accompanied by increasing the activity of caspases including caspase-8, caspase-9 and caspase-3. Consistent with enhancement of caspase activity, LSGE increased protein levels of cleaved caspase-3, caspase-8, caspase-9, and poly-ADP-ribose polymerase (PARP). These apoptotic effects of LSGE were inhibited by the pan-caspase inhibitor Z-VAD-fmk. These findings indicate that LSGE inhibits NCI-H460 human lung cancer cell growth by cell cycle arrest at the G0/G1 phase and induction of caspase-mediated apoptosis.

KW - Apoptosis induction

KW - Caspase activation

KW - G0/G1 phase arrest

KW - Human lung cancer cell

KW - Lipid-soluble ginseng extract

UR - https://www.scopus.com/pages/publications/79960243414

U2 - 10.1007/s11130-011-0232-6

DO - 10.1007/s11130-011-0232-6

M3 - Article

C2 - 21611769

AN - SCOPUS:79960243414

SN - 0921-9668

VL - 66

SP - 101

EP - 106

JO - Plant Foods for Human Nutrition

JF - Plant Foods for Human Nutrition

IS - 2

ER -

Lipid-Soluble Ginseng Extract Induces Apoptosis and G0/G1 Cell Cycle Arrest in NCI-H460 Human Lung Cancer Cells

Abstract

Bibliographical note

UN SDGs

Keywords

Access to Document

Fingerprint

Cite this