Lipid-Soluble Ginseng Extract Induces Apoptosis and G0/G1 Cell Cycle Arrest in NCI-H460 Human Lung Cancer Cells

Moo Rim Kang, Hwan Mook Kim, Jong Soon Kang, Kiho Lee, Sung Dong Lee, Dong Hoon Hyun, Man Jin In, Song Kyu Park, Dong Chung Kim

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

This study was performed to elucidate the anticancer mechanism of a lipid-soluble ginseng extract (LSGE) by analyzing induction of apoptosis and arrest of cell cycle progression using the NCI-H460 human lung cancer cell line. Proliferation of NCI-H460 cells was potently inhibited by LSGE in a dose-dependent manner. The cell cycle arrest at the G0/G1 phase in NCI-H460 cells was induced by LSGE. The percentage of G0/G1 phase cells significantly increased, while that of S phase cells decreased after treatment with LSGE. The expr.occurred through apoptosis, which was accompanied by increasing the activity of caspases including caspase-8, caspase-9 and caspase-3. Consistent with enhancement of caspase activity, LSGE increased protein levels of cleaved caspase-3, caspase-8, caspase-9, and poly-ADP-ribose polymerase (PARP). These apoptotic effects of LSGE were inhibited by the pan-caspase inhibitor Z-VAD-fmk. These findings indicate that LSGE inhibits NCI-H460 human lung cancer cell growth by cell cycle arrest at the G0/G1 phase and induction of caspase-mediated apoptosis.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalPlant Foods for Human Nutrition
Volume66
Issue number2
DOIs
StatePublished - Jun 2011

Keywords

  • Apoptosis induction
  • Caspase activation
  • G0/G1 phase arrest
  • Human lung cancer cell
  • Lipid-soluble ginseng extract

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