Lipid-Soluble Ginseng Extract Induces Apoptosis and G0/G1 Cell Cycle Arrest in NCI-H460 Human Lung Cancer Cells

Moo Rim Kang; Hwan Mook Kim; Jong Soon Kang; Kiho Lee; Sung Dong Lee; Dong Hoon Hyun; Man Jin In; Song Kyu Park; Dong Chung Kim

doi:10.1007/s11130-011-0232-6

Lipid-Soluble Ginseng Extract Induces Apoptosis and G0/G1 Cell Cycle Arrest in NCI-H460 Human Lung Cancer Cells

Moo Rim Kang, Hwan Mook Kim, Jong Soon Kang, Kiho Lee, Sung Dong Lee, Dong Hoon Hyun, Man Jin In, Song Kyu Park, Dong Chung Kim

Department of Life Science

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

This study was performed to elucidate the anticancer mechanism of a lipid-soluble ginseng extract (LSGE) by analyzing induction of apoptosis and arrest of cell cycle progression using the NCI-H460 human lung cancer cell line. Proliferation of NCI-H460 cells was potently inhibited by LSGE in a dose-dependent manner. The cell cycle arrest at the G0/G1 phase in NCI-H460 cells was induced by LSGE. The percentage of G0/G1 phase cells significantly increased, while that of S phase cells decreased after treatment with LSGE. The expr.occurred through apoptosis, which was accompanied by increasing the activity of caspases including caspase-8, caspase-9 and caspase-3. Consistent with enhancement of caspase activity, LSGE increased protein levels of cleaved caspase-3, caspase-8, caspase-9, and poly-ADP-ribose polymerase (PARP). These apoptotic effects of LSGE were inhibited by the pan-caspase inhibitor Z-VAD-fmk. These findings indicate that LSGE inhibits NCI-H460 human lung cancer cell growth by cell cycle arrest at the G0/G1 phase and induction of caspase-mediated apoptosis.

Original language	English
Pages (from-to)	101-106
Number of pages	6
Journal	Plant Foods for Human Nutrition
Volume	66
Issue number	2
DOIs	https://doi.org/10.1007/s11130-011-0232-6
State	Published - Jun 2011

Bibliographical note

Funding Information:
Acknowledgements This study was supported in part by a grant (No. A101836) of the Korean Health Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea and a grant from the KRIBB Research Initiative Program.

Keywords

Apoptosis induction
Caspase activation
G0/G1 phase arrest
Human lung cancer cell
Lipid-soluble ginseng extract

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11130-011-0232-6

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title = "Lipid-Soluble Ginseng Extract Induces Apoptosis and G0/G1 Cell Cycle Arrest in NCI-H460 Human Lung Cancer Cells",

abstract = "This study was performed to elucidate the anticancer mechanism of a lipid-soluble ginseng extract (LSGE) by analyzing induction of apoptosis and arrest of cell cycle progression using the NCI-H460 human lung cancer cell line. Proliferation of NCI-H460 cells was potently inhibited by LSGE in a dose-dependent manner. The cell cycle arrest at the G0/G1 phase in NCI-H460 cells was induced by LSGE. The percentage of G0/G1 phase cells significantly increased, while that of S phase cells decreased after treatment with LSGE. The expr.occurred through apoptosis, which was accompanied by increasing the activity of caspases including caspase-8, caspase-9 and caspase-3. Consistent with enhancement of caspase activity, LSGE increased protein levels of cleaved caspase-3, caspase-8, caspase-9, and poly-ADP-ribose polymerase (PARP). These apoptotic effects of LSGE were inhibited by the pan-caspase inhibitor Z-VAD-fmk. These findings indicate that LSGE inhibits NCI-H460 human lung cancer cell growth by cell cycle arrest at the G0/G1 phase and induction of caspase-mediated apoptosis.",

keywords = "Apoptosis induction, Caspase activation, G0/G1 phase arrest, Human lung cancer cell, Lipid-soluble ginseng extract",

author = "Kang, {Moo Rim} and Kim, {Hwan Mook} and Kang, {Jong Soon} and Kiho Lee and Lee, {Sung Dong} and Hyun, {Dong Hoon} and In, {Man Jin} and Park, {Song Kyu} and Kim, {Dong Chung}",

note = "Funding Information: Acknowledgements This study was supported in part by a grant (No. A101836) of the Korean Health Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea and a grant from the KRIBB Research Initiative Program.",

year = "2011",

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doi = "10.1007/s11130-011-0232-6",

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AU - Kim, Hwan Mook

AU - Kang, Jong Soon

AU - Lee, Kiho

AU - Lee, Sung Dong

AU - Hyun, Dong Hoon

AU - In, Man Jin

AU - Park, Song Kyu

AU - Kim, Dong Chung

N1 - Funding Information: Acknowledgements This study was supported in part by a grant (No. A101836) of the Korean Health Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea and a grant from the KRIBB Research Initiative Program.

PY - 2011/6

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N2 - This study was performed to elucidate the anticancer mechanism of a lipid-soluble ginseng extract (LSGE) by analyzing induction of apoptosis and arrest of cell cycle progression using the NCI-H460 human lung cancer cell line. Proliferation of NCI-H460 cells was potently inhibited by LSGE in a dose-dependent manner. The cell cycle arrest at the G0/G1 phase in NCI-H460 cells was induced by LSGE. The percentage of G0/G1 phase cells significantly increased, while that of S phase cells decreased after treatment with LSGE. The expr.occurred through apoptosis, which was accompanied by increasing the activity of caspases including caspase-8, caspase-9 and caspase-3. Consistent with enhancement of caspase activity, LSGE increased protein levels of cleaved caspase-3, caspase-8, caspase-9, and poly-ADP-ribose polymerase (PARP). These apoptotic effects of LSGE were inhibited by the pan-caspase inhibitor Z-VAD-fmk. These findings indicate that LSGE inhibits NCI-H460 human lung cancer cell growth by cell cycle arrest at the G0/G1 phase and induction of caspase-mediated apoptosis.

AB - This study was performed to elucidate the anticancer mechanism of a lipid-soluble ginseng extract (LSGE) by analyzing induction of apoptosis and arrest of cell cycle progression using the NCI-H460 human lung cancer cell line. Proliferation of NCI-H460 cells was potently inhibited by LSGE in a dose-dependent manner. The cell cycle arrest at the G0/G1 phase in NCI-H460 cells was induced by LSGE. The percentage of G0/G1 phase cells significantly increased, while that of S phase cells decreased after treatment with LSGE. The expr.occurred through apoptosis, which was accompanied by increasing the activity of caspases including caspase-8, caspase-9 and caspase-3. Consistent with enhancement of caspase activity, LSGE increased protein levels of cleaved caspase-3, caspase-8, caspase-9, and poly-ADP-ribose polymerase (PARP). These apoptotic effects of LSGE were inhibited by the pan-caspase inhibitor Z-VAD-fmk. These findings indicate that LSGE inhibits NCI-H460 human lung cancer cell growth by cell cycle arrest at the G0/G1 phase and induction of caspase-mediated apoptosis.

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KW - Caspase activation

KW - G0/G1 phase arrest

KW - Human lung cancer cell

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Lipid-Soluble Ginseng Extract Induces Apoptosis and G0/G1 Cell Cycle Arrest in NCI-H460 Human Lung Cancer Cells

Abstract

Bibliographical note

Keywords

UN SDGs

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