Lineage Specific Differentiation of Magnetic Nanoparticle-Based Size Controlled Human Embryoid Body

Boram Son, Jeong Ah Kim, Sungwoo Cho, Gun Jae Jeong, Byung Soo Kim, Nathaniel S. Hwang, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Human embryonic stem cells (hESCs) possess unique properties in terms of self-renewal and differentiation, which make them particularly well-suited for use in tissue engineering and regenerative medicine. The differentiation of hESCs in the form of human embryoid bodies (hEBs) recapitulates early embryonic development, and hEBs may provide useful insight into the embryological development of humans. Herein, cell-penetrating magnetic nanoparticles (MNPs) were utilized to form hEBs with defined sizes and the differentiation patterns were analyzed. Through intracellular delivery of MNPs into the hESCs, suspended and magnetized hESCs efficiently clustered in to hEBs driven by magnetic pin-based external magnetic forces. The hEB size was controlled by varying the suspended cell numbers that were applied in the magnetic pin system. After 3 days of differentiation in a suspended condition, ectodermal differentiation was observed to have been enhanced in the small hEBs (150 μm in diameter) while endodermal and mesodermal differentiation were enhanced in large hEBs (600 μm in diameter). This indicates that the size of the hEBs plays an important role in the early lineage commitment of hESCs, and MNP-based control of the hEB size would be a novel, useful methodology for lineage-specific hESC differentiation.

Original languageEnglish
Pages (from-to)1719-1729
Number of pages11
JournalACS Biomaterials Science and Engineering
Volume3
Issue number8
DOIs
StatePublished - 14 Aug 2017

Bibliographical note

Publisher Copyright:
© 2017 American Chemical Society.

Keywords

  • EB size control
  • embryoid bodies
  • human embryonic stem cells
  • lineage-specific differentiation
  • magnetic nanoparticles

Fingerprint

Dive into the research topics of 'Lineage Specific Differentiation of Magnetic Nanoparticle-Based Size Controlled Human Embryoid Body'. Together they form a unique fingerprint.

Cite this