Leukemic cell line, KG-1 has a functional loss of hOGG1 enzyme due to a point mutation and 8-hydroxydeoxyguanosine can kill KG-1

Jin Won Hyun, Jeong Yun Choi, Hui Hui Zeng, Yun Sil Lee, Hyun Sook Kim, Sun Hee Yoon, Myung Hee Chung

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

We tested the cytotoxic action of 8-hydroxyguanine (8ohG) by observing the viability of several leukemic cell lines (KG-1, U937, Jurkat and K 562) in the presence of 8-hydroxyguanosine (8ohdG), a nucleoside of 8ohG. It was found that 8ohdG showed cytotoxic action only to KG-1 and that only KG-1 showed a homozygous arginine 209 to glutamine mutation in the hOGG1 gene with an almost negligible hOGG1 enzyme activity. Possibly, the selective cytotoxicity in 8ohdG to KG-1 may be due to its low capacity to cope with an increase in the 8ohdG level in DNA resulting from the incorporation of 8ohdG present in the culture media. The mutational impairment of hOGG1 in KG-1 is the first report in leukemic cell lines. Using KG-1 with impaired hOGG1, we demonstrated cytotoxicity of 8ohdG probably due to its incorporation into cellular DNA. This new property of KG-1 may allow it to serve as an useful tool for studies of OGG1, oxidative DNA damage and the cytotoxic action of 8ohG.

Original languageEnglish
Pages (from-to)4476-4479
Number of pages4
JournalOncogene
Volume19
Issue number39
DOIs
StatePublished - 14 Sep 2000

Keywords

  • 8-Hydroxyguanine
  • Cytotoxicity
  • HOGG1
  • KG-1
  • Mutation

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