Large scale single cell analysis using high density hydrodynamic trapping arrays

Dino Di Carlo, Nima Aghdam, Paul J. Hung, Luke P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

This paper reports a novel approach for performing large scale single cell analysis which has significant advantages over miniaturized flow cytometry and laser scanning cytometry (LSC). Regular trapping arrays allow for high density analysis and ease image processing. Moreover, on-chip sample preparation (e.g. fluorescent labeling, washing) is performed, as opposed to manual intensive operations of incubation, centrifugation, and resuspension in previous techniques - saving time and reagents. Additionally, time-dependent phenomena of a large number of single cells over different time scales are characterized using this device. This method is well-suited to quantitative systems biology and rapid medical diagnostics.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages379-381
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: 9 Oct 200513 Oct 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Conference

Conference9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/TerritoryUnited States
CityBoston, MA
Period9/10/0513/10/05

Keywords

  • Cell array
  • Cytoenzymology
  • Quantitative biology
  • Single cell trapping

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