Label-free, single-cell cytotoxin kinetics assay by differential impedance spectroscopy

Daniele Malleo; J. Tanner Nevill; Luke P. Lee; Hywel Morgan

Label-free, single-cell cytotoxin kinetics assay by differential impedance spectroscopy

Daniele Malleo, J. Tanner Nevill, Luke P. Lee, Hywel Morgan

Department of Chemistry & Nanoscience

Research output: Contribution to conference › Paper › peer-review

Abstract

We present a device used to study the cytotoxic effects of Streptolysin-O (SLO) on single cells with differential impedance spectroscopy. A differential measurement is made to avoid background noise due to changes in temperature, pH, conductivity. This label-free microfluidic platform can be used to monitor transient response to toxin exposure continuously over long time periods. Upon exposure to SLO, captured HeLa cells exhibited exponential decays in the impedance response with time constants inversely proportional to the toxin concentration. The final toxin packing density into each cell was extrapolated from the impedance response to approximately 14 pores/μm ².

Original language	English
Pages	336-338
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 12 Oct 2008 → 16 Oct 2008

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	12/10/08 → 16/10/08

Keywords

Impedance spectroscopy
Label-free
Single-cell
Toxin

Cite this

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title = "Label-free, single-cell cytotoxin kinetics assay by differential impedance spectroscopy",

abstract = "We present a device used to study the cytotoxic effects of Streptolysin-O (SLO) on single cells with differential impedance spectroscopy. A differential measurement is made to avoid background noise due to changes in temperature, pH, conductivity. This label-free microfluidic platform can be used to monitor transient response to toxin exposure continuously over long time periods. Upon exposure to SLO, captured HeLa cells exhibited exponential decays in the impedance response with time constants inversely proportional to the toxin concentration. The final toxin packing density into each cell was extrapolated from the impedance response to approximately 14 pores/μm 2.",

keywords = "Impedance spectroscopy, Label-free, Single-cell, Toxin",

author = "Daniele Malleo and {Tanner Nevill}, J. and Lee, {Luke P.} and Hywel Morgan",

year = "2008",

language = "English",

pages = "336--338",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

}

Label-free, single-cell cytotoxin kinetics assay by differential impedance spectroscopy. / Malleo, Daniele; Tanner Nevill, J.; Lee, Luke P. et al.
2008. 336-338 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Label-free, single-cell cytotoxin kinetics assay by differential impedance spectroscopy

AU - Malleo, Daniele

AU - Tanner Nevill, J.

AU - Lee, Luke P.

AU - Morgan, Hywel

PY - 2008

Y1 - 2008

N2 - We present a device used to study the cytotoxic effects of Streptolysin-O (SLO) on single cells with differential impedance spectroscopy. A differential measurement is made to avoid background noise due to changes in temperature, pH, conductivity. This label-free microfluidic platform can be used to monitor transient response to toxin exposure continuously over long time periods. Upon exposure to SLO, captured HeLa cells exhibited exponential decays in the impedance response with time constants inversely proportional to the toxin concentration. The final toxin packing density into each cell was extrapolated from the impedance response to approximately 14 pores/μm 2.

AB - We present a device used to study the cytotoxic effects of Streptolysin-O (SLO) on single cells with differential impedance spectroscopy. A differential measurement is made to avoid background noise due to changes in temperature, pH, conductivity. This label-free microfluidic platform can be used to monitor transient response to toxin exposure continuously over long time periods. Upon exposure to SLO, captured HeLa cells exhibited exponential decays in the impedance response with time constants inversely proportional to the toxin concentration. The final toxin packing density into each cell was extrapolated from the impedance response to approximately 14 pores/μm 2.

KW - Impedance spectroscopy

KW - Label-free

KW - Single-cell

KW - Toxin

UR - http://www.scopus.com/inward/record.url?scp=84902477799&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902477799

SP - 336

EP - 338

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Label-free, single-cell cytotoxin kinetics assay by differential impedance spectroscopy

Abstract

Conference

Keywords

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Cite this