Label-free fluorescent real-time monitoring of adenylyl cyclase

Hyun Woo Rhee; Kyoung Shim Kim; Pyung Lim Han; Jong In Hong

doi:10.1016/j.bmcl.2009.12.018

Label-free fluorescent real-time monitoring of adenylyl cyclase

Hyun Woo Rhee, Kyoung Shim Kim, Pyung Lim Han, Jong In Hong

Divison of Brain & Cognitive Sciences

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

In cellular signaling, adenylyl cyclase plays a key role in the hydrolysis of ATP to cyclic AMP and pyrophosphate. Using a synthetic fluorescent chemosensor (PyDPA) which binds strongly to the pyrophosphate group, we have developed a label-free fluorescent real-time detection system for adenylyl cyclase. This assay would be the first adenylyl cyclase assay based on chemosensing the production of pyrophosphate.

Original language	English
Pages (from-to)	1145-1147
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	20
Issue number	3
DOIs	https://doi.org/10.1016/j.bmcl.2009.12.018
State	Published - 1 Feb 2010

Bibliographical note

Funding Information:
This work was supported by a NRF Grant funded by the MEST (Grant No. 2009-0080734 ) and Seoul R&BD. H.-W.R. is the recipient of the POSCO TJ Park Postdoctoral fellowship.

Keywords

Adenylyl cyclase
Cyclic AMP
Fluorescent chemosensor
Pyrophosphate
Real-time monitoring

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10.1016/j.bmcl.2009.12.018

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title = "Label-free fluorescent real-time monitoring of adenylyl cyclase",

abstract = "In cellular signaling, adenylyl cyclase plays a key role in the hydrolysis of ATP to cyclic AMP and pyrophosphate. Using a synthetic fluorescent chemosensor (PyDPA) which binds strongly to the pyrophosphate group, we have developed a label-free fluorescent real-time detection system for adenylyl cyclase. This assay would be the first adenylyl cyclase assay based on chemosensing the production of pyrophosphate.",

keywords = "Adenylyl cyclase, Cyclic AMP, Fluorescent chemosensor, Pyrophosphate, Real-time monitoring",

author = "Rhee, \{Hyun Woo\} and Kim, \{Kyoung Shim\} and Han, \{Pyung Lim\} and Hong, \{Jong In\}",

note = "Funding Information: This work was supported by a NRF Grant funded by the MEST (Grant No. 2009-0080734 ) and Seoul R\&BD. H.-W.R. is the recipient of the POSCO TJ Park Postdoctoral fellowship. ",

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doi = "10.1016/j.bmcl.2009.12.018",

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AU - Rhee, Hyun Woo

AU - Kim, Kyoung Shim

AU - Han, Pyung Lim

AU - Hong, Jong In

N1 - Funding Information: This work was supported by a NRF Grant funded by the MEST (Grant No. 2009-0080734 ) and Seoul R&BD. H.-W.R. is the recipient of the POSCO TJ Park Postdoctoral fellowship.

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Y1 - 2010/2/1

N2 - In cellular signaling, adenylyl cyclase plays a key role in the hydrolysis of ATP to cyclic AMP and pyrophosphate. Using a synthetic fluorescent chemosensor (PyDPA) which binds strongly to the pyrophosphate group, we have developed a label-free fluorescent real-time detection system for adenylyl cyclase. This assay would be the first adenylyl cyclase assay based on chemosensing the production of pyrophosphate.

AB - In cellular signaling, adenylyl cyclase plays a key role in the hydrolysis of ATP to cyclic AMP and pyrophosphate. Using a synthetic fluorescent chemosensor (PyDPA) which binds strongly to the pyrophosphate group, we have developed a label-free fluorescent real-time detection system for adenylyl cyclase. This assay would be the first adenylyl cyclase assay based on chemosensing the production of pyrophosphate.

KW - Adenylyl cyclase

KW - Cyclic AMP

KW - Fluorescent chemosensor

KW - Pyrophosphate

KW - Real-time monitoring

UR - https://www.scopus.com/pages/publications/74049099138

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SP - 1145

EP - 1147

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 3

ER -

Label-free fluorescent real-time monitoring of adenylyl cyclase

Abstract

Bibliographical note

Keywords

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