Label-free fluorescent real-time monitoring of adenylyl cyclase

Hyun Woo Rhee, Kyoung Shim Kim, Pyung Lim Han, Jong In Hong

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


In cellular signaling, adenylyl cyclase plays a key role in the hydrolysis of ATP to cyclic AMP and pyrophosphate. Using a synthetic fluorescent chemosensor (PyDPA) which binds strongly to the pyrophosphate group, we have developed a label-free fluorescent real-time detection system for adenylyl cyclase. This assay would be the first adenylyl cyclase assay based on chemosensing the production of pyrophosphate.

Original languageEnglish
Pages (from-to)1145-1147
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Issue number3
StatePublished - 1 Feb 2010

Bibliographical note

Funding Information:
This work was supported by a NRF Grant funded by the MEST (Grant No. 2009-0080734 ) and Seoul R&BD. H.-W.R. is the recipient of the POSCO TJ Park Postdoctoral fellowship.


  • Adenylyl cyclase
  • Cyclic AMP
  • Fluorescent chemosensor
  • Pyrophosphate
  • Real-time monitoring


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