TY - JOUR
T1 - Label-free emission assay of mercuric ions using DNA duplexes of poly(dT)
AU - Oh, Byul Nim
AU - Park, Sangji
AU - Ren, Jun
AU - Jang, Yoon Jung
AU - Kim, Seog K.
AU - Kim, Jinheung
PY - 2011/6/28
Y1 - 2011/6/28
N2 - In this study, an assay to quantify the presence of mercuric ions and methyl mercury by double-stranded DNA containing a poly(dT) sequence was developed using a light switch compound, Ru(phen)2(dppz)2+ (1), which is known to intercalate into double-stranded DNA. Upon treatment with mercuric ions, the metal-to-ligand charge transfer (MLCT) emission derived from the intercalation of 1 was reduced due to the formation of DNA duplexes containing T-Hg2+-T base pairs by the dehybridization of poly(dT)-poly(dA) duplexes at room temperature. As the concentration of Hg 2+ was increased, the emission of 1 gradually decreased. This label-free method had a detection limit of 5 nM. Other metal ions, such as K+, Ag+, Ca2+, Mg2+, Zn 2+, Mn2+, Co2+, Ni2+, Cu 2+, Cd2+, Cr3+, Fe3+, had no significant effect on reducing emission. This emission method can differentiate matched and mismatched poly(dT) sequences based on the dehybridization rate of dsDNA and the rate decreased in the order of T10C·A 11 ∼ T10A·A11 > T 10G·A11 > T11·A11.
AB - In this study, an assay to quantify the presence of mercuric ions and methyl mercury by double-stranded DNA containing a poly(dT) sequence was developed using a light switch compound, Ru(phen)2(dppz)2+ (1), which is known to intercalate into double-stranded DNA. Upon treatment with mercuric ions, the metal-to-ligand charge transfer (MLCT) emission derived from the intercalation of 1 was reduced due to the formation of DNA duplexes containing T-Hg2+-T base pairs by the dehybridization of poly(dT)-poly(dA) duplexes at room temperature. As the concentration of Hg 2+ was increased, the emission of 1 gradually decreased. This label-free method had a detection limit of 5 nM. Other metal ions, such as K+, Ag+, Ca2+, Mg2+, Zn 2+, Mn2+, Co2+, Ni2+, Cu 2+, Cd2+, Cr3+, Fe3+, had no significant effect on reducing emission. This emission method can differentiate matched and mismatched poly(dT) sequences based on the dehybridization rate of dsDNA and the rate decreased in the order of T10C·A 11 ∼ T10A·A11 > T 10G·A11 > T11·A11.
UR - http://www.scopus.com/inward/record.url?scp=79958780961&partnerID=8YFLogxK
U2 - 10.1039/c1dt10083a
DO - 10.1039/c1dt10083a
M3 - Article
C2 - 21562667
AN - SCOPUS:79958780961
SN - 1477-9226
VL - 40
SP - 6494
EP - 6499
JO - Dalton Transactions
JF - Dalton Transactions
IS - 24
ER -