Label-free detection of leukemic myeloblasts in hyaluronic acid

Suhyun Park, Hyueyun Kim, Minna Woo, Minsuk Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Chronic myeloid leukemia is generally required bone marrow biopsy for diagnosis. Although examining peripheral blood is less invasive, it has not been fully validated as a routine diagnostic test due to suboptimal sensitivity. To overcome this limitation, a number of methodologies based on microfluidics have been developed for sorting circulating tumor cells from peripheral blood of patients with leukemia. In order to develop a more convenient method, we designed an analysis protocol using motion microscopy that amplifies cellular micro motions in a captured video by re-rendering pixels to generate extreme magnified visuals. Intriguingly, no fluctuations around leukemic myeloblasts were observed with a motion microscope at any wavelength of 0–10 Hz. However, use of 0.05% hyaluronic acid, one type of non-newtonian fluid, demonstrated fluctuations around leukemic myeloblasts under conditions of 25 μm/s and 0.5–1.5 Hz with a motion microscope. Thus, the non-invasive detection of leukemic myeloblasts can offer a valuable supplementary diagnostic tool for assessment of drug efficacy for monitoring patients with chronic myeloid leukemia.

Original languageEnglish
Article number29
JournalJournal of Biological Engineering
Volume16
Issue number1
DOIs
StatePublished - Dec 2022

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant (2020R1A5A2019210 and 2019R1C1C1003384) funded by the Korean government (MSIT).

Publisher Copyright:
© 2022, The Author(s).

Keywords

  • Microfluidic system
  • Motion microscopy
  • Myeloid leukemia
  • Vibration

Fingerprint

Dive into the research topics of 'Label-free detection of leukemic myeloblasts in hyaluronic acid'. Together they form a unique fingerprint.

Cite this