Korean Sources metagenome library Korean sources

Hae Chul Park; So Ra Sung; Dong Gwan Kim; Bon Sung Koo; Byeongmoon Jeong; Jinheung Kim; Moon Young Yoon

Korean Sources metagenome library Korean sources

Translated title of the contribution: Pepper blight disease inhibition metagenome clone screening using soil metagenome library

Hae Chul Park, So Ra Sung, Dong Gwan Kim, Bon Sung Koo, Byeongmoon Jeong, Jinheung Kim, Moon Young Yoon

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

Abstract

We have purified Phytophthora capsici alpha and beta tubulin from Escherchia coli BL21(DE3). The recombinant alpha and beta tubulins were assembled into microtubule in vitro with specific conditions. The metagenome library was isolated from soil in the Mt. Yeo-Ki, Suwon, Korea and manufactured with the method mentioned in experiment contents for in vitro screening of microtubule assembly screening. FRET effect was used for microtubule assembly inhibitor screening with metagenome library. We got 2 metagenome clones from in vitro screening, and these 2 hit clones showed P. capsici growth inhibition activity on the growing pepper plants. These results suggest that new development of potent inhibitor for pepper blight disease and new approach to prevention of pepper blight disease.

Translated title of the contribution	Pepper blight disease inhibition metagenome clone screening using soil metagenome library
Original language	Korean
Pages (from-to)	228-231
Number of pages	4
Journal	Korean Journal of Microbiology
Volume	45
Issue number	2
State	Published - Jun 2009

Keywords

FRET
Metagenome
Pepper blight disease
Phytophthora capsici

Cite this

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title = "Korean Sources metagenome library Korean sources",

abstract = "We have purified Phytophthora capsici alpha and beta tubulin from Escherchia coli BL21(DE3). The recombinant alpha and beta tubulins were assembled into microtubule in vitro with specific conditions. The metagenome library was isolated from soil in the Mt. Yeo-Ki, Suwon, Korea and manufactured with the method mentioned in experiment contents for in vitro screening of microtubule assembly screening. FRET effect was used for microtubule assembly inhibitor screening with metagenome library. We got 2 metagenome clones from in vitro screening, and these 2 hit clones showed P. capsici growth inhibition activity on the growing pepper plants. These results suggest that new development of potent inhibitor for pepper blight disease and new approach to prevention of pepper blight disease.",

keywords = "FRET, Metagenome, Pepper blight disease, Phytophthora capsici",

author = "Park, {Hae Chul} and Sung, {So Ra} and Kim, {Dong Gwan} and Koo, {Bon Sung} and Byeongmoon Jeong and Jinheung Kim and Yoon, {Moon Young}",

year = "2009",

month = jun,

language = "Korean",

volume = "45",

pages = "228--231",

journal = "Korean Journal of Microbiology",

issn = "0440-2413",

publisher = "Microbiological Society of Korea",

number = "2",

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TY - JOUR

T1 - Korean Sources metagenome library Korean sources

AU - Park, Hae Chul

AU - Sung, So Ra

AU - Kim, Dong Gwan

AU - Koo, Bon Sung

AU - Jeong, Byeongmoon

AU - Kim, Jinheung

AU - Yoon, Moon Young

PY - 2009/6

Y1 - 2009/6

N2 - We have purified Phytophthora capsici alpha and beta tubulin from Escherchia coli BL21(DE3). The recombinant alpha and beta tubulins were assembled into microtubule in vitro with specific conditions. The metagenome library was isolated from soil in the Mt. Yeo-Ki, Suwon, Korea and manufactured with the method mentioned in experiment contents for in vitro screening of microtubule assembly screening. FRET effect was used for microtubule assembly inhibitor screening with metagenome library. We got 2 metagenome clones from in vitro screening, and these 2 hit clones showed P. capsici growth inhibition activity on the growing pepper plants. These results suggest that new development of potent inhibitor for pepper blight disease and new approach to prevention of pepper blight disease.

AB - We have purified Phytophthora capsici alpha and beta tubulin from Escherchia coli BL21(DE3). The recombinant alpha and beta tubulins were assembled into microtubule in vitro with specific conditions. The metagenome library was isolated from soil in the Mt. Yeo-Ki, Suwon, Korea and manufactured with the method mentioned in experiment contents for in vitro screening of microtubule assembly screening. FRET effect was used for microtubule assembly inhibitor screening with metagenome library. We got 2 metagenome clones from in vitro screening, and these 2 hit clones showed P. capsici growth inhibition activity on the growing pepper plants. These results suggest that new development of potent inhibitor for pepper blight disease and new approach to prevention of pepper blight disease.

KW - FRET

KW - Metagenome

KW - Pepper blight disease

KW - Phytophthora capsici

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M3 - Article

AN - SCOPUS:70349569952

SN - 0440-2413

VL - 45

SP - 228

EP - 231

JO - Korean Journal of Microbiology

JF - Korean Journal of Microbiology

IS - 2

ER -

Korean Sources metagenome library Korean sources

Abstract

Keywords

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