Keratinocyte-derived laminin-332 protein promotes melanin synthesis via regulation of tyrosine uptake

Heesung Chung, Hyejung Jung, Jung Hyun Lee, Hye Yun Oh, Ok Bin Kim, Inn Oc Han, Eok Soo Oh

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Melanocytes, which produce the pigment melanin, are known to be closely regulated by neighboring keratinocytes. However, how keratinocytes regulate melanin production is unclear. Here we report that melanin production in melanoma cells (B16F10 and MNT-1) was increased markedly on a keratinocyte-derived extracellular matrix compared with a melanoma cell-derived extracellular matrix. siRNA-mediated reduction of keratinocyte-derived laminin-332 expression decreased melanin synthesis in melanoma cells, and laminin-332, but not fibronectin, enhanced melanin content and α-melanocyte-stimulating hormone-regulated melanin production in melanoma cells. Similar effects were observed in human melanocytes. Interestingly, however, laminin-332 did not affect the expression or activity of tyrosinase. Instead, laminin-332 promoted the uptake of extracellular tyrosine and, subsequently, increased intracellular levels of tyrosine in both melanocytes and melanoma cells. Taken together, these data strongly suggest that keratinocyte-derived laminin-332 contributes to melanin production by regulating tyrosine uptake.

Original languageEnglish
Pages (from-to)21751-21759
Number of pages9
JournalJournal of Biological Chemistry
Volume289
Issue number31
DOIs
StatePublished - 1 Aug 2014

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