JSAP1 is required for the cell adhesion and spreading of mouse embryonic fibroblasts

Hee Jung Chae, Hye Yeong Ha, Joo Young Im, Ji Young Song, Sungmi Park, Pyung Lim Han

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The roles of JSAP1 and JIP1 in cell adhesion and spreading were examined using mouse embryonic fibroblasts (MEFs) deficient in JIP1 (JIP1-KO), JSAP1 (JSAP1-KO), and in both JIP1 and JSAP1 (double-KO), and by using their wild type. After being plated on fibronectin-coated culture plates, wild type MEFs rapidly adhered and differentiated to typical longitudinal fibroblasts in 4 h. JSAP1-KO MEFs showed a similar sequence of adhesion and cell spreading, but their adhesion was weak, and cell spreading sequence proceeded in a delayed manner compared with the wild type. In spreading JSAP1-KO MEFs, adhesion-triggered actin cytoskeleton reorganization and FAK activation proceeded at a slower pace than in wild type MEFs. The cellular properties of double-KO MEFs and JIP1-KO MEFs were similar to those of JSAP1-KO MEFs and wild type MEFs, respectively. These results suggest that JSAP1 plays a role in adhesion and cell spreading by regulating the rapid reorganization of the actin cytoskeleton.

Original languageEnglish
Pages (from-to)809-816
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - 30 Jun 2006

Bibliographical note

Funding Information:
This work was supported by Korea Research Foundation Grant (KRF-2003-015-E00167) for P.-L. Han.


  • Actin filaments
  • Cell adhesion
  • FAK
  • Scaffold protein


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