JNK pathway is required for retinoic acid-induced neurite outgrowth of human neuroblastoma, SH-SY5Y

Young Mi Yu, Pyung Lim Han, Ja Kyeong Lee

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Neurite outgrowth is a central event of neuronal differentiation that proceeds in multiple processes requiring various cellular factors. Here we demonstrated that c-Jun N-terminal kinase I (JNKI) plays an essential role in RA-induced neurite outgrowth of SH-SY5Y cells. Treatment of SH-SY5Y cells with RA induced a strong activation of JNKI within 10 min, and the immediate increase of JNKI activity returned to the basal level in an hour. The second surge of JNKI activity was observed around I day after RA treatment, which coincided with the period of extensive neurite outgrowth. Interestingly, phospho-JNK was concentrated in the nucleus of cells during the early induction, whereas it was distributed into neurite processes during the delayed second activation period. In SH-SY5Y carrying a dominant negative form of SEKI, an upstream kinase of JNKI, both early and late inductions of JNKI activity were repressed along with RA-induced neurite outgrowth. These results suggest that JNKI plays an essential role in RA-induced neuronal differentiation of SH-SY5Y cells.

Original languageEnglish
Pages (from-to)941-945
Number of pages5
JournalNeuroReport
Volume14
Issue number7
DOIs
StatePublished - May 2003

Keywords

  • JNKI
  • Neuronal differentiation
  • RA
  • SH-SY5Y

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