Isolation and proteomic analysis of a Chlamydomonas reinhardtii mutant with enhanced lipid production by the gamma irradiation method

Jaewon Baek, Jong Il Choi, Hyun Park, Sangyong Lim, Si Jae Park

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Abstract

In this study, an enhanced lipid-producing mutant strain of the microalga Chlamydomonas reinhardtii was developed by gamma irradiation. To induce the mutation, C. reinhardtii was gamma irradiated at a dose of 400 Gy. After irradiation, the surviving cells were stained with Nile red. The mutant (Cr-4013) accumulating 20% more lipid than the wild type was selected. Thin-layer chromatography revealed the triglyceride and free fatty acid contents to be markedly increased in Cr-4013. The major fatty acids identified were palmitic acid, oleic acid, linoleic acid, and linolenic acid. Random amplified polymeric DNA analysis showed partial genetic modifications in Cr-4013. To ascertain the changes of protein expression in the mutant strain, two-dimensional electrophoresis was conducted. These results showed that gamma radiation could be used for the development of efficient microalgal strains for lipid production.

Original languageEnglish
Pages (from-to)2066-2075
Number of pages10
JournalJournal of Microbiology and Biotechnology
Volume26
Issue number12
DOIs
StatePublished - Dec 2016

Keywords

  • Chlamydomonas reinhardtii
  • Lipid content
  • Mutant
  • Proteome

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