TY - JOUR
T1 - Isolation and characterization of microsatellite markers for the endangered crab species Sesarmops intermedius
AU - Lee, Sang kyu
AU - Jung, Jongwoo
AU - Kang, Seunghyun
AU - Kim, Won
N1 - Funding Information:
Acknowledgments This project was supported by a grant from the Eco-Technopia 21 Project funded by the Ministry of Environment, Korea and a grant from the Marine Biotechnology Programme funded by the Ministry of Land, Transport and Maritime Affairs of Korean government.
PY - 2012/3
Y1 - 2012/3
N2 - Sesarmops intermedius, which is designated as an endangered species in Korea, has been severely threatened by habitat destruction and pollution. From this species, we isolated 9 polymorphic microsatellites, which are independent of each other and represent allele frequencies under the Hardy-Weinberg equilibrium, except for 1 locus. The number of alleles per locus ranged from 7 to 20. The expected and observed heterozygosities ranged from 0.77 to 0.96 and from 0.63 to 0.95, respectively. We also examined cross-species amplification of the 9 developed microsatellite loci in 2 other crabs designated as endangered species in Korea and 6 sesarmid crabs, which live in habitats similar to that of S. intermedius. These markers have the potential to contribute to establishing conservation plans for this endangered crab species.
AB - Sesarmops intermedius, which is designated as an endangered species in Korea, has been severely threatened by habitat destruction and pollution. From this species, we isolated 9 polymorphic microsatellites, which are independent of each other and represent allele frequencies under the Hardy-Weinberg equilibrium, except for 1 locus. The number of alleles per locus ranged from 7 to 20. The expected and observed heterozygosities ranged from 0.77 to 0.96 and from 0.63 to 0.95, respectively. We also examined cross-species amplification of the 9 developed microsatellite loci in 2 other crabs designated as endangered species in Korea and 6 sesarmid crabs, which live in habitats similar to that of S. intermedius. These markers have the potential to contribute to establishing conservation plans for this endangered crab species.
KW - Cross-species amplification
KW - Microsatellite markers
KW - Semi-terrestrial crab
KW - Sesarmops intermedius
UR - http://www.scopus.com/inward/record.url?scp=84859826198&partnerID=8YFLogxK
U2 - 10.1007/s12686-011-9504-6
DO - 10.1007/s12686-011-9504-6
M3 - Article
AN - SCOPUS:84859826198
SN - 1877-7252
VL - 4
SP - 183
EP - 185
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 1
ER -