Silkworm (Bombyx mori) hemolymph showed an apoptosis-inhibiting activity in insect cells (Sf 9) infected with baculovirus (AcNPV). The addition of silk-worm hemolymph into the culture medium increased the host cell longevity due to its apoptosis-inhibition activity. Components with an apoptosis-inhibiting effect were purified from the silkworm hemolymph by heat treatment, gel-filtration chromatography, and ion-exchange chromatography. The component with highest activity was characterized by periodic acid-Schiff staining, isoelectric focusing, MALDI-TOF-mass spectrometry, and N-terminal sequencing and was found to be a nonglycosylated monomeric protein with a molecular weight of ca. 28,000 Da.
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - 2001|
Bibliographical noteFunding Information:
The authors acknowledge the financial support of the Korea Science & Engineering Foundation through the Nano Bio-Electronic & System Center. This work was supported in part by the Brain Korea 21 Program from the Ministry of Education. Silkworms were kindly provided by Dr. Sam-Eun Kim, Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Suwon, Korea.