Iron promotes the survival and neurite extension of serum-starved PC12 cells in the presence of NGF by enhancing cell attachment

Jin Hee Hong, Kyung Min Noh, Young Eun Yoo, So Young Choi, Sun Young Park, Yoon Hee Kim, Jun Mo Chung

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Delayed death of serum-starved PC12 cells on a poly-L-lysine (PLL) matrix was observed, even in the presence of NGF. NGF blocked the apoptotic death of attached but not detached cells, which suggests that delayed death may be related to cell detachment from the PLL matrix. Iron selectively blocked this anoikis-like death by increasing cell attachment. Interestingly, the addition of > 10 μM FeCl2 to the culture medium generated gelatinous iron precipitates, and the removal of the precipitates abolished the iron effect. Attachment experiments using poly-HEMA supported the role of iron precipitates on cell-to-matrix adhesion. The expression of integrin β1, neither N-cadherin nor α/β-catenin, was also significantly increased by iron. In addition to its effect on cell viability, iron promoted the outgrowth of neuntes. Our results collectively indicate that iron functions as a necessary co-element for NGF by enhancing cell attachment, survival, and neurite extension.

Original languageEnglish
Pages (from-to)10-19
Number of pages10
JournalMolecules and Cells
Volume15
Issue number1
StatePublished - Feb 2003

Keywords

  • Cell Attachment
  • Iron
  • Neurite Outgrowth
  • NGF
  • PC12 Cells

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