Intraspecific hybridization of Anopheles sinensis (Diptera: Culicidae) strains from Thailand and Korea

Gi Sik Min, Wej Choochote, Atchariya Jitpakdi, Se Joo Kim, Won Kim, Jongwoo Jung, Anuluck Junkum

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Anopheles (Anopheles) sinensis [Wiedemann (1828)] is a member of the hyrcanus species group, and it has been incriminated as the natural or experimental malaria vectors in the Republic of Korea, Japan, China, and Indonesia. In Thailand, however, An. sinensis seems to be of little medical importance. Hybridization tests among the three iso-female lines (isolines) of An. sinensis [i.e., Form A (X, Y1) and Form B (X, Y2) (Thailand strain), and Form B (X, Y2) (Korean strain)] were established based on two distinct types of metaphase chromosomes and geographical differences. The chromosomal form of the Korean strain was first identified from this study. Results of reciprocal and back crosses indicated that both karyotypic forms of the An. sinensis Thailand and Korean strains were genetically compatible, and provided viable progenies and completely synaptic polytene chromosomes. The sequences of the rDNA internal-transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit II (COII) among the An. sinensis strains were nearly identical to each other, and the intraspecific sequence variability was very low (0.0-0.6%). Sequence comparisons among the cryptic inter-species (i.e., An. sinensis, An. lesteri, and An. yatsiishiroensis), however, revealed extensive divergence, and the intraspecific variability ranged from 12.2 to 34.6%. Therefore, it is concluded from these results and previous vector ability studies that the An. sinensis Forms A and B exhibit cytological polymorphic races that have different vector abilities in their transmission of malaria, depending on their geographical locations.

Original languageEnglish
Pages (from-to)198-204
Number of pages7
JournalMolecules and Cells
Issue number2
StatePublished - Oct 2002


  • Anopheles sinensis
  • Cytochrome c Oxidase Subunit II
  • Internal Transcribed Spacer 2
  • Intraspecific Hybridization
  • Karyotypic Forms


Dive into the research topics of 'Intraspecific hybridization of Anopheles sinensis (Diptera: Culicidae) strains from Thailand and Korea'. Together they form a unique fingerprint.

Cite this