Intracellular Remodeling and Accumulation of Aberrant Lysosomes in Differentiation of Tonsil-Derived Mesenchymal Stem Cells into Parathyroid-Like Cells

Young Il Jo, Gyungah Kim, Yoon Mi Jin, Yoon Jeong Park, Han Su Kim, Yoon Shin Park

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Differentiation of mesenchymal stem cells (MSC) into a variety of cell lineages such as adipocytes, osteocytes, and chondrocytes is often accompanied up-regulation of autophagy. In our study, we demonstrated that the expression of autophagy-associated proteins (p-Beclin 1, LC3A, LC3B, p-AMPK, p-mTOR and ATG3, ATG7, and ATG12-5) over a period of time was hardly distinguishable from control tonsil-derived MSC (TMSC). Despite the unnoticeable difference in autophagy activation between differentiated TMSC (dTMSC) and the control (cTMSC), we reported significant changes in intracellular compositions in differentiated TMSC into functional parathyroid-like cells secreting parathyroid hormone (PTH). By using transmission electron microscopy (TEM), we observed accumulation of multivesicular bodies (MVB) comprising small, degraded compartments densely accumulated as dark granular or amorphous clumps, multilamellar bodies and lipid droplets in dTMSC. However, no such structures were found in cTMSC. These results suggest that differentiation of TMSC into parathyroid-like cells producing PTH hormone is hardly dependent on autophagy activation in the beginning of our conditions. Furthermore, our results of intracellular remodeling and accumulated endo-lysosomal storage bodies in the later stages of TMSC differentiation present a possible role of the structures in PTH secretion.

Original languageEnglish
Pages (from-to)411-420
Number of pages10
JournalTissue Engineering and Regenerative Medicine
Issue number4
StatePublished - 1 Aug 2017

Bibliographical note

Funding Information:
This study was supported in part by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning NRF-2013R1A1A3007591 and 2017R1A2B4002611 by the Grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI14C-1557). This work was supported by the research grant of the Chungbuk National University in 2016.

Publisher Copyright:
© 2017, The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht.


  • Autophagy
  • Multilamellar bodies
  • Multivesicular bodies
  • Parathyroid-like differentiation
  • Tonsil-derived mesenchymal stem cells


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