Interaction proteome analysis of Xanthomonas Hrp proteins

Mi Jang, Byoung Chul Park, Do Hee Lee, Kwang Hee Bae, Sayeon Cho, Hyun Seok Park, Baek Rak Lee, Sung Goo Park

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Because of the importance of the type III protein-secretion system in bacteria-plant interaction, its function in bacterial pathogenesis of plants has been intensively studied. To identify bacterial proteins interacting with Xanthomonas hrp gene products that are involved in pathogenicity, we performed the glutathione-bead binding analysis of Xanthomonas lysates containing GST-tagged Hrp proteins. Analysis of glutathione-bead bound proteins by 1-DE and MALDI-TOF has demonstrated that Avr proteins, RecA, and several components of the type III secretion system interact with HrpB protein. This proteomic approach could provide a powerful tool in finding interaction partners of Hrp proteins whose roles in host-pathogen interaction need further studies.

Original languageEnglish
Pages (from-to)359-363
Number of pages5
JournalJournal of Microbiology and Biotechnology
Volume17
Issue number2
StatePublished - Feb 2007

Keywords

  • 1-DE
  • Interaction protein
  • MALDITOF
  • Pathogenesis
  • hrp gene

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