Integrated system investigating shear-mediated platelet interactions with von Willebrand factor using microliters of whole blood

Bryan Lincoln, Antonio J. Ricco, Nigel J. Kent, Lourdes Basabe-Desmonts, Luke P. Lee, Brian D. MacCraith, Dermot Kenny, Gerardene Meade

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


We report an integrated platelet translocation analysis system that measures complex dynamic platelet-protein surface interactions in microliter volumes of unmodified anticoagulated whole blood under controlled fluid shear conditions. The integrated system combines customized platelet-tracking image analysis with a custom-designed microfluidic parallel plate flow chamber and defined von Willebrand factor surfaces to assess platelet trajectories. Using a position-based probability function that accounts for image noise and preference for downstream movement, outputs include instantaneous and mean platelet velocities, periods of motion and stasis, and bond dissociation kinetics. Whole blood flow data from healthy donors at an arterial shear rate (1500s-1) show mean platelet velocities from 8.9±1.0 to 12±4μms-1. Platelets in blood treated with the antiplatelet agent c7E-Fab fragment spend more than twice as much time in motion as platelets from untreated control blood; the bond dissociation rate constant (koff) increases 1.3-fold, whereas mean translocation velocities do not differ. Blood from healthy unmedicated donors was used to assess flow assay reproducibility, donor variability, and the effects of antiplatelet treatment. This integrated system enables reliable, rapid populational quantification of platelet translocation under pathophysiological vascular fluid shear using as little as 150μl of blood.

Original languageEnglish
Pages (from-to)174-183
Number of pages10
JournalAnalytical Biochemistry
Issue number2
StatePublished - Oct 2010

Bibliographical note

Funding Information:
This material is based on works supported by the Science Foundation Ireland under Grant 05/CE3/B754 .


  • GPIb-IX-V
  • Microfluidic parallel-plate flow chamber
  • Platelet translocation
  • Position-based probability function
  • Von Willebrand factor


Dive into the research topics of 'Integrated system investigating shear-mediated platelet interactions with von Willebrand factor using microliters of whole blood'. Together they form a unique fingerprint.

Cite this