Integrated microfluidic platform with nanoplasmonic aptasensor for cancer research

Hansang Cho; Yolanda Zhang; Brian Lee; Audrey Kimteng; Jane P. Bearinger; Brian R. Baker; Ted A. Laurence; Stephen M. Lane; Luke P. Lee

Integrated microfluidic platform with nanoplasmonic aptasensor for cancer research

Hansang Cho
, Yolanda Zhang
, Brian Lee
, Audrey Kimteng
, Jane P. Bearinger
, Brian R. Baker
, Ted A. Laurence
, Stephen M. Lane
, Luke P. Lee

Department of Chemistry & Nanoscience

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

In this paper, we propose an integrated microfluidic platform utilizing aptamer and surface plasmonics for a long-term and real-time assessment and label-free detection of secreted growth factors under the spatial and temporal control of a chemically simulated tumor microenvironment. The integrated platform achieved detection of vascular endothelial growth factor (VEGF) down to 1 nM in buffer solution and also VEGF secreted from MCF-7 (human breast cancer) cells upon continuous stimulation with 0.1 mM estrodiole for 37 hrs. The integrated microfluidic platform may be useful for the application to cell-based screening and high-throughput investigation of drug candidates that inhibit VEGF secretion.

Original language	English
Title of host publication	Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1482-1484
Number of pages	3
ISBN (Print)	9780979806421
State	Published - 2009
Event	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of Duration: 1 Nov 2009 → 5 Nov 2009

Publication series

Name	Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/Territory	Korea, Republic of
City	Jeju
Period	1/11/09 → 5/11/09

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Aptamer
Breast cancer
Integration
Microfluidics
Plasmonics

Cite this

Cho, H., Zhang, Y., Lee, B., Kimteng, A., Bearinger, J. P., Baker, B. R., Laurence, T. A., Lane, S. M., & Lee, L. P. (2009). Integrated microfluidic platform with nanoplasmonic aptasensor for cancer research. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1482-1484). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Cho, Hansang ; Zhang, Yolanda ; Lee, Brian et al. / Integrated microfluidic platform with nanoplasmonic aptasensor for cancer research. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 1482-1484 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

@inproceedings{f7d01e57e84d41179a616e36f190ff08,

title = "Integrated microfluidic platform with nanoplasmonic aptasensor for cancer research",

abstract = "In this paper, we propose an integrated microfluidic platform utilizing aptamer and surface plasmonics for a long-term and real-time assessment and label-free detection of secreted growth factors under the spatial and temporal control of a chemically simulated tumor microenvironment. The integrated platform achieved detection of vascular endothelial growth factor (VEGF) down to 1 nM in buffer solution and also VEGF secreted from MCF-7 (human breast cancer) cells upon continuous stimulation with 0.1 mM estrodiole for 37 hrs. The integrated microfluidic platform may be useful for the application to cell-based screening and high-throughput investigation of drug candidates that inhibit VEGF secretion.",

keywords = "Aptamer, Breast cancer, Integration, Microfluidics, Plasmonics",

author = "Hansang Cho and Yolanda Zhang and Brian Lee and Audrey Kimteng and Bearinger, \{Jane P.\} and Baker, \{Brian R.\} and Laurence, \{Ted A.\} and Lane, \{Stephen M.\} and Lee, \{Luke P.\}",

year = "2009",

language = "English",

isbn = "9780979806421",

series = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "1482--1484",

booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

note = "13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",

}

Cho, H, Zhang, Y, Lee, B, Kimteng, A, Bearinger, JP, Baker, BR, Laurence, TA, Lane, SM & Lee, LP 2009, Integrated microfluidic platform with nanoplasmonic aptasensor for cancer research. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 1482-1484, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 1/11/09.

Integrated microfluidic platform with nanoplasmonic aptasensor for cancer research. / Cho, Hansang; Zhang, Yolanda; Lee, Brian et al.
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 1482-1484 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Integrated microfluidic platform with nanoplasmonic aptasensor for cancer research

AU - Cho, Hansang

AU - Zhang, Yolanda

AU - Lee, Brian

AU - Kimteng, Audrey

AU - Bearinger, Jane P.

AU - Baker, Brian R.

AU - Laurence, Ted A.

AU - Lane, Stephen M.

AU - Lee, Luke P.

PY - 2009

Y1 - 2009

N2 - In this paper, we propose an integrated microfluidic platform utilizing aptamer and surface plasmonics for a long-term and real-time assessment and label-free detection of secreted growth factors under the spatial and temporal control of a chemically simulated tumor microenvironment. The integrated platform achieved detection of vascular endothelial growth factor (VEGF) down to 1 nM in buffer solution and also VEGF secreted from MCF-7 (human breast cancer) cells upon continuous stimulation with 0.1 mM estrodiole for 37 hrs. The integrated microfluidic platform may be useful for the application to cell-based screening and high-throughput investigation of drug candidates that inhibit VEGF secretion.

AB - In this paper, we propose an integrated microfluidic platform utilizing aptamer and surface plasmonics for a long-term and real-time assessment and label-free detection of secreted growth factors under the spatial and temporal control of a chemically simulated tumor microenvironment. The integrated platform achieved detection of vascular endothelial growth factor (VEGF) down to 1 nM in buffer solution and also VEGF secreted from MCF-7 (human breast cancer) cells upon continuous stimulation with 0.1 mM estrodiole for 37 hrs. The integrated microfluidic platform may be useful for the application to cell-based screening and high-throughput investigation of drug candidates that inhibit VEGF secretion.

KW - Aptamer

KW - Breast cancer

KW - Integration

KW - Microfluidics

KW - Plasmonics

UR - https://www.scopus.com/pages/publications/84901813950

M3 - Conference contribution

AN - SCOPUS:84901813950

SN - 9780979806421

T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 1482

EP - 1484

BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009

Y2 - 1 November 2009 through 5 November 2009

ER -

Cho H, Zhang Y, Lee B, Kimteng A, Bearinger JP, Baker BR et al. Integrated microfluidic platform with nanoplasmonic aptasensor for cancer research. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 1482-1484. (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Integrated microfluidic platform with nanoplasmonic aptasensor for cancer research

Abstract

Publication series

Conference

UN SDGs

Keywords

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Cite this