Insulin-sensitizing activities of tanshinones, diterpene compounds of the root of Salvia miltiorrhiza Bunge

Seung Hee Jung, Hee Jung Seol, Su Jin Jeon, Kun Ho Son, Jong Ran Lee

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

In this study, the effects of the extract and four tanshinone compounds from the dried root of Salvia miltiorrhiza Bunge (Labiatae) on the tyrosine phosphorylation of the insulin receptor (IR) β-subunit and the downstream signaling were examined in Chinese-hamster ovary cells expressing human insulin receptors (CHO/IR cells) as well as in 3T3-L1 adipocytes. In addition the translocation of the glucose transporter 4 was investigated in 3T3-L1 adipocytes. Total extract of Danshen (1-10 μg/ml) and the four tanshinones (10 μM) did not show any activity, but the total extract and the tanshinone I, IIA and 15, 16-dihydrotanshinone I except cryptotanshinone enhanced the activity of insulin (1 nM) on the tyrosine phosphorylation of the IR as well as the activation of the downstream kinases Akt, ERK1/2, and GSK3β. In the adipocytes the same IR-downstream signaling and the translocation of glucose transporter 4 were demonstrated by the three tanshinones in the presence of insulin. These insulin-sensitizing activities of tanshinones may be useful for developing a new class of specific IR activators as anti-diabetic agents.

Original languageEnglish
Pages (from-to)327-335
Number of pages9
JournalPhytomedicine
Volume16
Issue number4
DOIs
StatePublished - Apr 2009

Keywords

  • 3T3-L1-adipocytes
  • Glucose transporter
  • Insulin receptor tyrosine phosphorylation
  • Insulin signal transduction
  • Salvia miltiorrhiza
  • Tanshinones

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